IndraLab

Statements

MYC binds USP37. 38 / 39
4 | 1 7 26
sparser
"USP37‐C‐Myc interactions might play an essential role in keloid pathogenesis, which could be used to design small molecules and peptidyl disruptors."
36333840
sparser
"The previously reported deubiquiting enzyme USP37 can directly bind to and deubiquitinate c-Myc, thereby stabilizing c-Myc ( Pan et al., 2015 )."
34102342
sparser
"Interestingly, unlike USP28 [ xref ], USP37 directly binds to c-Myc and acts independently of Fbw37."
34201062
trips
"Further studies indicate that USP37 directly interacts with c-Myc and deubiquitinates c-Myc in a DUB activity-dependent manner."
25284584
sparser
"USP37 interacts directly with MYC to promote its deubiquitination and maintain its protein stability ( xref )."
35836933
biogrid
No evidence text available
25284584
biogrid
No evidence text available
25284584
biogrid
No evidence text available
25284584
biogrid
No evidence text available
25284584
sparser
"Further studies indicate that USP37 directly interacts with c-Myc and deubiquitinates c-Myc in a DUB activity-dependent manner."
25284584
reach
"For example, Usp9x binds and stabilizes Mcl1, and Usp37 interacts with and deubiquitinates Myc [97,98] ."
26459133
sparser
"We further overexpressed myc-USP37 in U2OS cells and performed antigen capture using PCNA antibody, which was subsequently probed with myc antibody, and we observed an interaction between these two proteins (Fig.  xref E)."
37118828
sparser
"Given the structural properties of the interaction sites in the c-MYC-USP37 complex, a peptidyl inhibitor has been designed."
36985413
sparser
"Dissociation constant and free energy of the c-MYC-USP37 molecular complex was predicted to be 1.9 × 10 −8 Kda and −8.1 KJ, respectively."
36985413
reach
"Predicting c-MYC and USP37 Interaction."
36985413
sparser
"Disrupting c-MYC-USP37 Complex."
36985413
reach
"Since stabilization of c-MYC by USP37 is unwanted, disrupting the physical interaction between c-MYC and USP37 has therapeutic potential."
36985413
sparser
"Since the molecular interactions between c-MYC and USP37 are majorly driven by the intrinsically disordered regions in both proteins, instead of a small molecule, a peptidyl disruptor was designed."
36985413
reach
"Since the molecular interactions between c-MYC and USP37 are majorly driven by the intrinsically disordered regions in both proteins, instead of a small molecule, a peptidyl disruptor was designed."
36985413
reach
"This indicates that the predicted c-MYC USP37 complex is structurally plausible.The interaction between c-MYC and USP37 leads to post translational stabilization of the c-MYC, resulting in the abnormal increase in the half-life of the molecule, which is estimated to be 30 min in normal cells [54]."
36985413
reach
"Since the interacting region of c-MYC for USP37 is structurally disordered, it may not be a suitable target for small molecule inhibitor and/or disruptor [55]; rather, a peptidyl disruptor could be used to block the interaction between USP37 and c-MYC."
36985413
sparser
"HADDOCK prediction showed that peptide in majority of the simulations interacted with the c-MYC to the regions targeted (USP37 binding sites) and sits well within the cavity of c-MYC that binds with USP37."
36985413
sparser
"Therefore, it is plausible to conceive that in the ABC subtype of DLBCL the c-MYC may also interact with USP37, resulting in its stabilization."
36985413
sparser
"Moreover, interaction of c-MYC with USP37 is expected to remove ubiquitin tag from the protein via its catalytically active C19 domain."
36985413
sparser
"This indicates that the predicted c-MYC USP37 complex is structurally plausible."
36985413
sparser
"The interaction between c-MYC and USP37 leads to post translational stabilization of the c-MYC, resulting in the abnormal increase in the half-life of the molecule, which is estimated to be 30 min in normal cells [ xref ]."
36985413
sparser
"This retention of c-MYC with in cell leads to abnormal growth of the cells, and therefore, the interaction sites of c-MYC-USP37 complex could be targeted to prevent increased accumulation of c-MYC within the cells."
36985413
reach
"Further studies indicate that USP37 directly interacts with c-Myc and deubiquitinates c-Myc in a DUB activity dependent manner."
25284584
sparser
"This revealed that purified GST-USP37 bound to FLAG-CDC73 ( xref B) and purified GST-CDC73 bound to Myc-USP37 ( xref C) in E. coli strain BL21."
35742816
sparser
"Since the interacting region of c-MYC for USP37 is structurally disordered, it may not be a suitable target for small molecule inhibitor and/or disruptor [ xref ]; rather, a peptidyl disruptor could be used to block the interaction between USP37 and c-MYC."
36985413
sparser
"Reciprocal binding between CDC73 and USP37 was also detected in co-IP experiments performed in COS7 cells using FLAG-CDC73 and Myc-USP37 ( xref B)."
35742816
sparser
"Nearly 80% of the residues of c-MYC that interacts with USP37 have been found interacting with the designed peptide, suggesting that the peptide is specific to c-MYC-USP37 complex and could be used for targeted therapy against the ABC subtype of DLBCL."
36985413
sparser
"To see if CDC73 and USP37 colocalized in the cell, FLAG-CDC73 and Myc-USP37 vectors were simultaneously transfected into COS7 cells."
35742816
sparser
"After co-transfection of FLAG-CDC73 and Myc-USP37 expression vectors in COS7 cells, expression of CDC73 and USP37 proteins, respectively, was observed, thus showing that CDC73 and USP37 were co-expressed at the same location in the cell nucleus ( xref D)."
35742816
sparser
"Alanine scans of both the consensus regions showed inhibition in the interaction between c-MYC and USP37 when the residues Tyr177, Gln179, Asp180, Cys188, Asp251, Glu253, Glu254 and Glu255 were substituted with alanine ( xref D,E)."
36985413
sparser
"Predicting c-MYC and USP37 Interaction."
36985413
sparser
"Since stabilization of c-MYC by USP37 is unwanted, disrupting the physical interaction between c-MYC and USP37 has therapeutic potential."
36985413
sparser
"C-MYC-USP37 Intermolecular Complexes."
36985413