IndraLab

Statements

P3 binds STAMBP. 8 / 8
| 8
sparser
"To gain insight into the orientation of AMSH with respect to SH3, we used different AMSH peptide sequence containing either the P 239 (AMSH-P3) or the 230 PP 231 (AMSH-P4) motif."
27725184
sparser
"First, the UIM-SH3 CSP mapping shows different intensities with AMSH-P3 and -P4, especially for residues Tyr218 and Asp219 (see Fig. 2 c and d)."
27725184
sparser
"Second, the 1 H, 15 N-HSQC signals corresponding to the interaction between UIM-SH3 and AMSH-P3 are in the fast exchange regime compared to the UIM-SH3/AMSH-P4 complex that underwent signals in the in[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27725184
sparser
"Third, the directions of the shifts in the 1 H, 15 N-HSQC spectra of UIM-SH3 upon titration by AMSH-P3 and -P4 are similar (Figs."
27725184
sparser
"While AMSH-P3 and -P4 have similar dissociation constants upon binding to SH3 (see Table 1 ), the dissociation rate constant for the UIM-SH3/AMSH-P3 complex rose significantly to 3900 ± 1500s − 1 (res[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27725184
sparser
"In the light of these observations, it is likely that the P 239 residue of AMSH-P3 would be oriented in a direction close to the residues Trp246 and Glu224 of SH3, while 230 PP 231 of AMSH-P4 would po[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27725184
sparser
"These results, which added to the fact that AMSH-P3 still binds to SH3, indicate that the Lys 238 residue has a profound effect on the binding of AMSH with SH3."
27725184
sparser
"While 231 PVVDRSLKP 239 is the sequence that would maintain AMSH in the correct orientation with respect to STAM, the removal of 228 AKPP 231 (AMSH-P3) only slightly affects the binding affinity while[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27725184