IndraLab

Statements

USP28 binds ∆NP63. 7 / 7
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"Therefore, we were able to show that the USP28‐∆Np63 axis is implicated in the regulation and control of the expression of SCC marker genes as well as genes controlling cell fate and SCC identity."
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"While the interaction between USP28 and ∆Np63 is independent of the catalytic site within the DUB, our results show that the active cysteine at position 171 is essential to facilitate the stabilizing effect on the ∆Np63 protein."
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"The ∆Np63‐USP28 axis is required to maintain the identity of SCC cells."
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"To investigate a therapeutic potential of targeting the USP28‐∆Np63 axis in SCC cells of different origins, we used a set of human cancer cell lines, comprising the pancreas lines PANC‐1 (ADC) and BXPC‐3 (SCC); cervical cancer cell lines HeLa (ADC), SiHa and Ca Ski (SCC); the head‐and‐neck cell line Detroit 562 (SCC); and the lung cell lines H1299 (ADC) and LUDLU‐1 adh (SCC)."
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"Finally, we targeted the USP28‐∆Np63 axis via AZ1 in SCC cell lines and compared proliferative responses to a respective ADC line (Figs  xref G and H, and xref D)."
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"Alternatively, SCC tumors express high levels of USP28 and the oncogenic transcription factors NOTCH1, c-JUN, c-MYC, and ∆Np63 ( xref B). squamous tumors depend on the USP28-∆Np63 axis to maintain SCC cell identity and induce the formation of tumors in vivo."
| PMC
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"To assess whether the observed genetic alterations are attributed to the USP28‐∆Np63 axis in SCC, we next analysed “Hallmark” gene sets of reported USP28 substrates NOTCH1, MYC and AP1‐c‐JUN, in USP28 and ∆Np63 knock‐down A‐431 cells (Fig  xref J and K)."
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