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"In addition, GST pull-down assays were performed to validate the in vitro results showing that GST-USP3 effectively decreased COL9A3 and COL6A5 recombinant proteins in AGS cells."

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"USP3-GST fusion protein were expressed in BL21 competent cells and purified with Glutathione sepharose 4B. Ubiquitinated p53 protein was incubated with recombinant USP3 in the deubiquitination buffer [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"