IndraLab

Statements


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sparser
"Positive colonies were streaked out onto Cam plates and grown at 37 °C. Clones were grown in Cam-LB media for glycerol stocks and subsequent large preparations of HSV2 gD-BAC DNA using the Large Construct kit (Qiagen Inc.)."

sparser
"To remove the co-integrated Kan cassette from the inserts, in 1 ml LB-Cam broth medium, E. coli cells carrying recombinant MVA-BAC were inoculated at 32 °C and 220 rpm for 2 h until the culture became cloudy."

sparser
"Clones were grown in Cam-LB media for glycerol stocks and subsequent large preparations of HSV2 gD-BAC DNA using the Large Construct kit (Qiagen Inc.)."

sparser
"Thereafter, to induce the production of I- Sce I, 1 ml pre-warmed LB-Cam medium + 2% arabinose was added to the culture followed by 1 h incubation at 32 °C and 220 rpm."

sparser
"After 2–3 h incubation at 32 °C in a shaker incubator, the cells were screened on a LB-Cam agar plate supplemented with 1% arabinose."