IndraLab

Statements

CALM1 binds dlb. 13 / 13
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"Blue colonies expressing the lacZ β-galactosidase were observed and selected on LB-CAM plates including X-gal and IPTG."
38583808
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"Thereafter, to induce the production of I- Sce I, 1 ml pre-warmed LB-Cam medium + 2% arabinose was added to the culture followed by 1 h incubation at 32 °C and 220 rpm."
31399106
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"Transformed cells were spread on LB-Cam agar, and after 16 h at 30 °C, the resulting lawn of colonies was recovered."
18272177
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"After 2–3 h incubation at 32 °C in a shaker incubator, the cells were screened on a LB-Cam agar plate supplemented with 1% arabinose."
31399106
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"The recovered cells of the epPCR library were regrown in LB-Cam to mid-log phase, washed in 1× Vogel–Bonner salts and plated on the minimal selection medium containing 50 μM IPTG and Cam."
18272177
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"35 After 48 h of incubation at 30 °C, the 40 fastest-growing colonies were used to inoculate the LB-Cam medium (120 μL) and grown to saturation."
18272177
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"Cells were transformed with pCA24N- purF(1–04) plasmid DNA and used to inoculate an LB-Cam culture (40 mL) directly."
18272177
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"Dilutions plated on LB-Cam indicated that the initial inoculum was ∼ 60 transformants."
18272177
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"After 24 h of growth at 30 °C, the four largest colonies were picked, grown in LB-Cam and, in the case of variant purF(2–02) , sequenced using the primers pCA24N.for, pCA24N.rev2, pUC rev + 120, purF.[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18272177
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"In each case, a single colony was used to inoculate LB-Cam (400 μL) and grown to saturation."
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"To remove the co-integrated Kan cassette from the inserts, in 1 ml LB-Cam broth medium, E. coli cells carrying recombinant MVA-BAC were inoculated at 32 °C and 220 rpm for 2 h until the culture became cloudy."
31399106
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"Dilutions were also spread on LB-Cam agar, enabling us to estimate that approximately 147,000 transformed cells were plated on the selection medium."
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"35 Plates were incubated at 30 °C for 10 days, and colonies that appeared were grown in LB-Cam medium for storage and further analysis."
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