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Statements

STAMBP binds CHMP3. 68 / 73
23 2 | 19 24
sparser
"We show that AMSH binds to CHMP3 in 0 HBS (closed conformation) and 500 HBS (open conformation) with dissociation constants of 5.6 and 392 nM, respectively, implying that the closed conformer binds [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18395747
sparser
"However, there are two reasons why the CHMP3–AMSH interaction may induce CHMP3 opening as suggested."
18395747
sparser
"The high-affinity CHMP3–AMSH interaction may explain why AMSH overexpression renders full-length CHMP3 dominant negative with respect to HIV-1 budding, 9 because it may sequester CHMP3 in a state tha[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18395747
sparser
"Because CHMP3 within a functional ESCRT-III complex seems to act late in budding, the presence of a high-affinity CHMP3–AMSH interaction would ensure efficient deubiquitination before budding is compl[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18395747
sparser
"The ~ 60 nM K d for the CHMP3-AMSH interaction is to date the highest K d reported for an ESCRT-III MIM-MIT interaction."
21827950
sparser
"In principle, CHMP3 could interact with Vps4 via a similar region; this, however, raises the question as to how a high-affinity CHMP3–AMSH interaction switches to a low-affinity CHMP3–Vps4 interacti[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18395747
sparser
"Interaction constants characterizing the AMSH and CHMP3 protein–peptide interaction were determined by direct titration in 0 HBS, 150 HBS, and 500 HBS at 25 °C. The concentrations used are summariz[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18395747
sparser
"The high affinity interaction between CHMP3 and AMSH is mainly mediated by 6 salt bridges along the CHMP3 helix compared to 2 salt bridges in case of the spastin-MIT-CHMP1B interaction."
21827950
reach
"Collectively data from these three different interaction studies provide strong evidence that the predicted interactions between CHMP1B and AMSH, CHMP3 and AMSH, and CHMP2A and LOC129531 may well be p[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16730941
reach
"Although CHMP3 binds with high affinity to AMSH, ESCRT-III CHMP1A and B show only micromolar interactions."
21827950
reach
"Determination of a minimal AMSH and CHMP3 complex."
21827950
sparser
"Given this, an appropriate molecular model of the AMSH-CHMP3 complex is proposed, in which AMSH is first recruited to membranes early in the ESCRT pathway via ESCRT-0 STAM ( xref C) [ xref ]."
35883466
reach
"In a previous study, we found that AMSH binds the ESCRT-III subunit CHMP3 and plays a role in MVB/late endosomes [6] ."
21531206
reach
"Although the AMSH and CHMP3 interaction is conserved between Drosophila and mammals, our data show that in human cells AMSH binds to a distinct subset of ESCRT III components, with robust interactions[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16730941
reach
"Like PalB, AMSH interacts with CHMP3 (Vps24) and contains a single MIT domain."
19056728
biogrid
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29997244
biogrid
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16730941
sparser
"In a previous study, we found that AMSH binds the ESCRT-III subunit CHMP3 and plays a role in MVB/late endosomes [6] ."
21531206
sparser
"With the exception of the CHMP3-AMSH interaction that was reported to have a K d of about 60 nM xref , the measured affinities are usually in the range of 2 to 100 µM. The tight interaction between p60-MIT and p80-CTD can be explained by a large total surface area of 2774Å 2 buried upon complex formation as compared to, for example, 1238 Å 2 of the Vps4 MIT-Vps2 complex (K d 30 μM) xref , 1946 Å 2 of the spastin-CHMP1B complex (K d 12 μM) xref or only 543 Å 2 for the VPS4B-IST1 (K d 11 μM) complex xref ."
29097679
biogrid
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28514442
biogrid
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17159328
biogrid
No evidence text available
17261583
reach
"In contrast, the CHMP3 and AMSH complex structure is mainly stabilized by hydrogen bonds and salt bridges while hydrophobic contacts seem to play only a minor role."
29097679
sparser
"In order to understand the structural basis of the AMSH-CHMP3 interaction we defined an N-terminal AMSH domain (AMSHΔC) by proteolysis that allowed the isolation of a soluble complex with a C-terminal fragment of CHMP3."
21827950
biogrid
No evidence text available
17159328
biogrid
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26186194
biogrid
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16730941
biogrid
No evidence text available
17159328
biogrid
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16760479
biogrid
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33961781
biogrid
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16760479
sparser
"The structurally defined Vps4-MIT-MIM1 interaction ( xref , xref ) is a reasonable model for the AMSH MIT-CHMP3 interaction."
21332354
reach
"Similarly, the mammalian deubiquitinating enzyme AMSH binds directly to the ESCRT-III subunit Vps24, although it is not known if Vps24 mediates endosomal recruitment of this enzyme [30–34] ."
16716591
reach
"Furthermore, CHMP3 interacts with CHMP2 members (Morita and Sundquist, 2004) and AMSH, thus linking CHMP3 to the HRS and STAM complex (McCullough et al., 2006)."
16740483
biogrid
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25416956
hprd
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17711858
sparser
"A conserved interlog interaction between CHMP3 and AMSH was also observed in Drosophila melanogaster [28] and CHMP3 has been shown to form an in vitro complex with AMSH and STAM [34] ."
16730941
sparser
"86 AMSH operates in the ESCRT (endosomal sorting complexes required for transport) pathway, interacting with charged multivesicular body protein 3 (CHMP3) in the ESCRT-III complex, 93 mediating deubiq[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23856261
biogrid
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32296183
hprd
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16730941
biogrid
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17146056
reach
"Various reports in the field have consistently found a strong interaction between CHMP3 and AMSH, but have diverged with respect to interactions with other CHMPs."
21827939
sparser
"For example the MIT domains of AMSH and USP8 (also known as UBPY or UBP8), two endosomal de-ubiquitinating enzymes, have some partners in common but only AMSH can interact with CHMP3 [14] ."
19302785
reach
"It seems unlikely that AMSH can have a significant catalytic role with respect to hydrolyzing the last ubiquitin attached to the cargo, yet, AMSH binds to ESCRT-III component CHMP3 with relatively high affinity."
24151880
reach
"The structure reveals that the AMSH and CHMP3 complex is stabilized mainly by polar interactions, manifesting into tight binding between the two proteins, with a dissociation constant (K D) of 60 nM, the highest reported K D for an ESCRT-III MIM -MIT interaction."
24151880
reach
"A conserved interlog interaction between CHMP3 and AMSH was also observed in Drosophila melanogaster [28] and CHMP3 has been shown to form an in vitro complex with AMSH and STAM [34]."
16730941
reach
"CHMP1 (Did2), CHMP2 (Vps2) and CHMP3 (Vps24) can interact with two mammalian deubiquitinating enzymes, AMSH and UBPY, to facilitate their recruitment directly [XREF_BIBR, XREF_BIBR, XREF_BIBR]."
19143619
reach
"22 Although the C-terminal CHMP3 peptide shows a similar affinity for AMSH interaction, full-length CHMP3 interacts much tighter with AMSH revealing nanomolar dissociation constants (K d = 5.6 nM), th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18395747
sparser
"This suggests that the AMSH MIT domain interaction with CHMP3 differs substantially from the low-affinity Vps4 MIT domain interaction."
18395747
sparser
"Consistent with previous data, 9 CHMP3(9–183) lacking the C-terminal peptide region did not interact with AMSH(1–206) in the 50 HBS, 150 HBS, and 500 HBS buffer conditions using ITC."
18395747
biogrid
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18395747
sparser
"Because the C-terminal region carries a substantial negative charge, the NaCl concentration-dependent difference in K d suggests that the electrostatic interactions may play a role in CHMP3–AMSH int[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18395747
biogrid
No evidence text available
16431367
sparser
"We tested the interaction of mammalian Vps24 (mVps24) with AMSH and with a C-terminal truncated form, AMSH (1-284)."
16431367
biogrid
No evidence text available
17261583
sparser
"Trials to determine whether CHMP3 binds only to the AMSH MIT domain could not be performed because we were unable to obtain a soluble monodisperse form of the AMSH MIT domain."
18395747
biogrid
No evidence text available
16730941
biogrid
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17711858
reach
"The general MIT-MIM contacts range from mostly hydrophobic interactions observed in the VPS4-CHMP1A, Vps4p-Vps2p, VPS4B-CHMP6 and Saci1372 (Vps4 like)-Saci1373 (ESCRT-II-like) complexes [XREF_BIBR - XREF_BIBR, XREF_BIBR] to a mixture of polar and hydrophobic interactions in case of spastin MIT and CHMP1B [XREF_BIBR] to mostly polar contacts dominating the AMSH and CHMP3 complex."
21827950
reach
"Thus the structure of the AMSH and CHMP3 complex extends the diversity of MIT domain interaction surfaces for peptide ligands."
21827950
reach
"The high affinity interaction between CHMP3 and AMSH is mainly mediated by 6 salt bridges along the CHMP3 helix compared to 2 salt bridges in case of the spastin-MIT-CHMP1B interaction."
21827950
sparser
"In addition, the AMSH MIT domain interacts with the membrane-associated CHMP3 with a dissociation constant of ~ 60 nM, while its DUB activity could act in a radius of more than 20 nm [22] ."
27725184
biogrid
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19615732
biogrid
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17711858
biogrid
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16730941
reach
"22 Nevertheless, the structure may indicate how the C-termini of full-length CHMP proteins might interact with MIT domains via a larger surface, leading to substantially higher binding affinity.We sho[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18395747
sparser
"Various reports in the field have consistently found a strong interaction between CHMP3 and AMSH, but have diverged with respect to interactions with other CHMPs."
21827939
sparser
"Mutational analysis of the CHMP3–AMSH interaction implicated CHMP3 residues Arg216 and Leu217 in binding 9 ; both residues are part of the proposed Vps4–MIT–CHMP interaction motif."
18395747