IndraLab

Statements

OTUD3 inhibits mTORC1. 8 / 8
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"These findings partially confirm the essential role of the KPTN protein in OTUD3’s ability to downregulate the mTORC1 signaling pathway in cells."
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"3.6 OTUD3 suppressed proliferation and growth of HeLa by downregulating the mTORC1."
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"Under various amino acid starvation or stimulation conditions, overexpressing OTUD3 reduces mTORC1 signaling activation, while knocking out OTUD3 further enhances it."
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"3.3 OTUD3 downregulated mTORC1 signaling pathway on account of its enzymatic deubiquitinase activity."
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"To test this hypothesis, we transfected KPTN shRNA into PTEN-KO HeLa cells exogenously expressing OTUD3-Myc, observing whether OTUD3 could still inhibit the mTORC1 signaling pathway in the absence of both PTEN and KPTN."
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"The results showed that the ability of OTUD3 to inhibit the mTORC1 signaling pathway was significantly weakened under conditions of concurrent PTEN and KPTN depletion (Figure 4E)."
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"Furthermore, OTUD3’s downregulation of the mTORC1 pathway requires the presence of its substrate, KPTN."
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"This suggests that OTUD3C76A, lacking deubiquitinase activity, cannot reverse the upregulation of the mTORC1 signaling pathway caused by the knockout of endogenous OTUD3.In this section of the study, through a series of experiments involving amino acid starvation and stimulation in HeLa cells, we demonstrated that OTUD3 can downregulate the mTORC1 signaling pathway, and this downregulation is dependent on the enzymatic deubiquitinase activity of OTUD3."
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