IndraLab

Statements

UCHL3 binds POLD4. 10 / 10
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"Tumorsphere formation and in vitro limiting dilution assay were performed to investigate the effect of UCHL3-POLD4 on GSC self-renewal."
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"Apoptosis, TUNEL, cell cycle phase distribution, modification of the Single Cell Gel Electrophoresis (Comet), γ-H2AX immunofluorescence, and colony formation assays were conducted to evaluate the influence of UCHL3-POLD4 on GSC in ionizing radiation."
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"Both in vitro and in vivo assays indicated that targeted depletion of the UCHL3-POLD4 axis reduced GSC self-renewal and tumorigenic capacity and resistance to IR treatment by impairing homologous recombination (HR) and nonhomologous end joining (NHEJ)."
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"These findings reveal a new signaling axis for GSC PMT regulation and highlight UCHL3-POLD4 as a potential therapeutic target in GBM."
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"UCHL3 interacted with, depolyubiquitinated, and stabilized POLD4."
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"UCHL3 interacted with, stabilized and depolyubiquitylated POLD4."
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"Then, we confirmed these interactions by co-IP assay and GST pull-down assay and found that inactive UCHL3 (Flag-UCHL3 S75A) can bind POLD4 directly, indicating that the enzyme activity of UCHL3 was not crucial for their interaction (Fig. 5D, E)."
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"Then, we confirmed these interactions by co-IP assay and GST pull-down assay and found that inactive UCHL3 (Flag-UCHL3 S75A) can bind POLD4 directly, indicating that the enzyme activity of UCHL3 was not crucial for their interaction (Fig.  xref D, E)."
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"It has been established that the deubiquitinase UCHL3 binds and stabilizes p12 in GSCs."
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"It has been established that the deubiquitinase UCHL3 binds and stabilizes p12 in GSCs."
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