IndraLab

Statements

OTUD3 binds Flag. 7 / 7
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"Flag-OTUD3 and His-BHLHE22 vectors were co-transfected into 293 T cells to verify exogenous OTUD3 and BHLHE22 protein interaction (Fig.  xref d)."
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"Flag-OTUD3 WT or Flag-OTUD3 C76A , Myc-GRP78, and HA-Ub were transfected into HEK293T cells as indicated."
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"Flag-OTUD3 WT (wild-type) or Flag-OTUD3 C76A, Myc-GRP78 and HA-Ub were transfected into HEK293T cells as indicated."
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"Compared with the IgG control group, 17 proteins with a difference >4‐fold in the Flag‐OTUD3 group were initially screened as interacting proteins of OTUD3."
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"Additionally, we validated this interaction by reciprocal experiments, where we overexpressed KPTN-Myc and OTUD3-Flag and obtained similar results ( xref )."
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"Further confirmation involved separate overexpression of KPTN-Flag and OTUD3-Flag in HEK-293T cells, showing their interaction with endogenous KPTN and OTUD3, respectively ( xref )."
38288086
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"The western blot results showed protein bands bound to Flag‐OTUD3 (Figure  xref )."
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