IndraLab
Statements
sparser
"As positive control for nuclear localization, CFP-OTU1 was coexpressed with mRFP fused to a bipartite-type nuclear localization signal (NLS) derived from the Agrobacterium VirD2 protein ( xref ). xref B shows that, similarly to free mRFP, CFP-OTU1 accumulated in the cytoplasm—displaying transvacuolar strands (green arrowhead) and variations in cytosol thickness at the cell cortex ( xref , xref )—and in the nucleus (white arrowhead), colocalizing with mRFP-VirD2 NLS."
sparser
"We cannot rule out that at least some of the cytoplasmic signal of CFP-OTU1 derives from degradation of this fusion protein; however, usually, degradation of GFP-tagged proteins results in the loss of fluorescence, representing the rationale for degradation assays that utilize GFP fusions of the proteins of interest ( xref , xref , xref )."