IndraLab

Statements

NS2 binds OTUB1. 8 / 8
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"The result showed that V5-OTUB1 was coimmunoprecipitated with HA-NS2 ( xref , lane 4), demonstrating that OTUB1 interacts with NS2 in HEK293T cells."
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"In an LC-MS proteomic investigation, we identified a host DUB, OTUB1, which interacts with viral NS2."
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"We used a GST-pulldown assay to evaluate the interaction between OTUB1 and NS2 in vitro ."
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"The paradox in which OTUB1 appears to promote both an innate anti-IAV immune response as well as IAV proliferation can be explained by the results of our study, which shows that OTUB1 interacts with NS2 to set the stage for viral cRNA synthesis and is critical for vRNP nuclear export (Figs xref and xref )."
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"Thus, OTUB1 binds NS2 directly in vitro ."
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"Our study sheds light on the critical viral-host interaction between NS2 and OTUB1 that facilitates viral propagation (Figs xref and xref ), and further research into the changes in quantity and locality of OTUB1 following IAV infection may be warranted, in order to better understand how IAV utilizes host factors to enhance viral proliferation."
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"NS2 interacts with OTUB1."
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"To verify the interaction between NS2 and OTUB1, we cotransfected HEK293T cells with plasmids that expressed HA-NS2 and Flag-OTUB1."
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