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"The relA mediated temperature sensitivity was osmoremedial and could be completely suppressed, for example, by the addition of NaCl to the medium at a concentration of 0.3 M."

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"First, an immunoblotting assay validated that lactate-treated proliferating BV2 and HMC3 cells led to elevated p–p65 level in relation to cells treated with Nacl, while proliferating BV2 or HMC3 cells simultaneously treated with JSH-23 reverse the increased p–p65 (Fig. 7A, B)."

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"Moreover, cell immunofluorescence also verified that lactate-treated proliferating BV2 and HMC3 cells enhanced the expression of activated p65 in nucleus compared to cells treated with NaCl, while lactate-treated proliferating BV2 and HMC3 cells simultaneously treated with JSH-23 reduced nuclear activation of p65, which is consistent with the results from the immunoblotting assay (Fig. 7C, D)."