IndraLab

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VCPIP1 activates PSMC3. 2 / 2
| 2

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"Furthermore, the Co-IP assay detected endogenous PSMC3 expression with the VCPIP1 overexpression but not with the empty vector control (Fig. 5B), and the interaction did not result from the possible increase of PSMC3 by VCPIP1 (Fig. 5C) and HBx (Fig. 5D)."

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"To determine if the 20S proteasome was the functional component for counteracting HBx stability via VCPIP1-mediated PSMC3, purified VCPIP1-Flag and PSMC3-Myc proteins were also prepared, and both were tagged with 6× His epitope (Fig. 6A)."