IndraLab

Statements

ACTN1 binds KCNA5. 6 / 6
| 6
sparser
"These results confirm the interaction between Kv1.5 and α-actinin-2 seen in the yeast two-hybrid growth assay, both in vitro and in HEK cells."
10812072
sparser
"To further investigate the binding of Kv1.5 to α-actinin-2, a GST fusion protein affinity binding method was employed."
11389904
sparser
"Thus, a significant portion of the interaction between Kv1.5 and α-actinin-2 is mediated across a region where the N-terminus meets the core transmembrane domains of the protein."
10812072
sparser
"None yielded lacZ reporter gene activity above control, showing that there is little to no interaction between the C-terminus of Kv1.5 and α-actinin-2."
10812072
sparser
"As shown in , neither Ca nor calmodulin had any effect on α-actinin-2 binding to Kv1.5."
11389904
sparser
"We utilized the yeast two-hybrid lacZ reporter gene to estimate the relative strength of interaction between various domains in Kv1.5 and the α-actinin-2 interacting fragment."
10812072