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CYLD activates (-)-noscapine. 15 / 15
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"Flow cytometry and immunofluorescence microscopy reveal that CYLD increases the ability of noscapine to induce mitotic arrest and apoptosis."
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"Based on the data presented in this work, we propose the following model for how CYLD promotes noscapine activity in leukemia cells."
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"We found that CYLD, but not its DeltaCG1 mutant, significantly promoted the effect of noscapine on microtubule assembly in vitro."
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"CYLD enhances noscapine activity to induce mitotic arrest and apoptosis in a microtubule dependent manner."
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"Examination of cellular microtubules as well as in vitro assembled microtubules shows that CYLD enhances the effect of noscapine on microtubule polymerization."
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"Immunofluorescence microscopy also showed that CYLD and its C/S mutant, but not its DeltaCG1 mutant, increased noscapine activity to cause mitotic arrest."
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"XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR CYLD has also been shown to stimulate noscapine activity in acute lymphoblastic leukemia via a microtubule dependent mechanism."
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"CYLD enhances the effect of noscapine on microtubule polymerization."
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"Thus, we examined whether CYLD modulates the ability of noscapine to cause mitotic arrest."
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"In addition, flow cytometric analysis of DNA content showed that CYLD and its C/S mutant, but not its DeltaCG1 mutant, promoted the ability of noscapine to induce the accumulation of G2/M cells."
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"To understand how CYLD modulates noscapine activity in leukemia cells, we transfected cells with GFP, GFP-CYLD, GFP-CYLD-DeltaCG1, in which the extreme N-terminal CAP-Gly domain of CYLD was deleted, or GFP-CYLD-C/S, in which cysteine 601 was mutated to serine to disrupt the deubiquitinase activity of CYLD."
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"It is possible that CYLD may also exert on these pathways to stimulate noscapine activity."
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"Here we demonstrate that cylindromatosis (CYLD), a microtubule associated tumor suppressor protein, modulates the activity of noscapine both in cell lines and in primary cells of acute lymphoblastic leukemia (ALL)."
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"Collectively, these results suggest that CYLD stimulates noscapine activity to promote microtubule polymerization, without affecting microtubule nucleation."
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"These data indicate that CYLD increases the effect of noscapine on microtubule polymerization."
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