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USP28 inhibits CLASP1. 5 / 5
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"Likewise, Annexin-V-positive cells were increased in USP28-KO clones compared with USP28-depleted cells reconstituted with USP28 or MAST1 (Fig. 5G), indicating that loss of USP28 sensitizes cells to cisplatin-mediated DNA damage and induced cell death.Finally, we wished to investigate the molecular mechanism of USP28 regulation on MAST1-driven cisplatin-resistance."
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"An anchorage-independent colony formation assay demonstrated that USP28-KO clones showed reduced colony numbers compared with the mock control, while the colony-forming ability was increased in USP28-depleted cells reconstituted with USP28 or MAST1 (Fig. 6A and Supplementary Fig. 15A)."
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"Similarly, cellular invasion and migration rate were significantly hindered in USP28-KO clones, whereas USP28-depleted cells reconstituted with USP28 or MAST1 showed reversed results (Fig. 6B, C and Supplementary Fig. 15B, C)."
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"Next, we measured the ability of USP28-depleted cells to impair tumor growth in NSG mice by subcutaneously injecting USP28-KO cells and USP28-depleted cells reconstituted with either USP28 or MAST1 obtained from Fig. 5A."
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"However, mice bearing USP28-depleted cells reconstituted with USP28 or MAST1 displayed increased tumor size, volume and weight (Fig. 6D, E, Supplementary Table S6)."
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