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Isopropyl β-d-thiogalactoside increases the amount of JOSD2. 1 / 1
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"After inoculation, the bacterial cells were grown at 37 °C to an optical density at 600 nm of 0.4–0.6 OD value, and expression of the GST-fusion JOSD2 was induced by 300 μmol/L isopropyl β-d-thiogalactoside (Selleck Chem, Houston, TX, USA) for 18 h at 25 °C."