IndraLab

Statements

VCPIP1 binds GEMIN4. 16 / 16
| 16
reach
"After incubation, the p97 bound to p47 was coimmunoprecipitated by anti-p47 antibodies in order to separate it from the p97 bound to VCIP135."
12473691
reach
"Although VCIP135 also binds to p97 [13], it remains unclear whether complex formation between VCIP135 and p97 is really necessary for p97 mediated membrane fusion."
23500464
reach
"We have shown that VCIP135 binds to syntaxin5 and disassembles the p97, p47, and syntaxin5 complex through p97 ATP hydrolysis."
12473691
reach
"VCIP135 binds to the p97, p47, and syntaxin-5 complex and can dissociate p97 and p47 from syntaxin-5."
15037600
reach
"During late mitosis, VCIP135 becomes dephosphorylated and associates with the p97, p47, and Syn5 complex."
28819009
reach
"Golgi WAC interacts with the deubiquitinase VCIP135, which binds VCP and p97."
26687599
reach
"VCPIP1 interacts with p97, a highly abundant protein implicated in a vast range of cellular activities including the endoplasmic reticulum associated degradation (ERAD) pathway and HIF1alpha stability XREF_BIBR."
19699092
reach
"Since the p97, VCIP135, and syntaxin5 complex is very unstable (XREF_FIG C), a tighter VCIP135 and p97 complex then dissociates from syntaxin5 (XREF_FIG F)."
12473691
reach
"However, the VCIP135, p97, p47, GST, and syn5DeltaTM complex was dissociated only in the presence of ATP (XREF_FIG E, lane 4), and the VCIP135 and p97 complex was observed in the supernatant (unpublished data)."
12473691
reach
"This provides a reasonable explanation for our biochemical data that p47 (271-370) binds to p97 but not to the p97 and VCIP135 complex (XREF_FIG B)."
12473691
reach
"Although VCIP135 can bind to p97 in cytosol (XREF_FIG A), VCIP135 bound to syntaxin5 does not form a complex with p97 (XREF_FIG C, lane 4)."
12473691
reach
"This suggests that the formation of a VCIP135 and p97 complex on syntaxin5 dissociates VCIP135 from syntaxin5."
12473691
reach
"Therefore, formation of the p97 and VCIP135 complex is thought to be required for p97 mediated Golgi membrane fusion."
23500464
reach
"In late mitosis, the DUB VCIP135, which associates with p97 via its UBX-L (UBX like) domain, deubiquitinates Syn5, which permits interaction between the SNAREs, membrane fusion and finally the formation of Golgi cisternae [XREF_BIBR]."
28819009
reach
"VCIP135 binds to the p97, p47, and SNARE complex and dissociates it via p97 catalyzed ATP hydrolysis (Uchiyama et al., 2002)."
17141156
reach
"We therefore investigated the effect of phosphorylation on the binding of VCIP135 to p97 or WAC.We first tested whether phosphorylated VCIP135 binds to p97."
23500464