IndraLab

Statements

USP35 binds MAVS. 23 / 25
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"Taken together, these results suggest that USP35 interacts with MAVS both in vivo and in vitro, and this interaction is regulated by viral stimulation."
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"To investigate the mechanism how USP35 inhibits the RIG-I-MAVS pathway, we examined the interaction between USP35 and MAVS."
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"Reciprocal Co-IP assay displayed that endogenous USP35 could bind to endogenous MAVS (Fig. 3D, E)."
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"To validate whether USP35 directly interacts with MAVS, we performed a GST Pull-down assay using the purified USP35 protein and detected a direct interaction between GST-USP35 and MAVS (Fig. 3F)."
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"To explore whether the binding of USP35 to MAVS is regulated by VSV virus stimulation, we infected cells with the VSV virus."
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"The interaction between USP35 and MAVS was enhanced in a time-dependent manner following VSV treatment (Fig. 3C)."
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"To identify the domains involved in the binding of USP35 to MAVS, we constructed a series of truncations of MAVS."
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"USP35 directly binds to MAVS and removes the K63-linked polyubiquitin chain of MAVS, a process dependent on the enzyme activity of USP35."
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"USP35 interacts with MAVS in vivo and in vitro."
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"To investigate the mechanism how USP35 inhibits the RIG-I-MAVS pathway, we examined the interaction between USP35 and MAVS."
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"Previous authors have demonstrated that USP35 negatively regulates STING in the DNA signaling pathway, and our current work demonstrates that USP35 also interacts with MAVS in the RNA signaling pathway."
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"Mechanistically, USP35 interacts with MAVS and removes its K63-linked polyubiquitin chains, thereby inhibiting viral-induced MAVS-TBK1-IRF3 activation and downstream inflammatory gene expression."
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"USP35 directly binds to MAVS and relies on its deubiquitinating enzyme activity."
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"In this study, Co-IP, GST-pull down and immunofluorescence experiments have shown that USP35 binds directly to MAVS."
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"USP35 directly binds to MAVS and relies on its deubiquitinating enzyme activity. thereby inhibiting its function. (Fig. xref )."
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"In this study, Co-IP, GST-pull down and immunofluorescence experiments have shown that USP35 binds directly to MAVS."
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"The interaction between USP35 and MAVS was enhanced in a time-dependent manner following VSV treatment (Fig. xref )."
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"To identify the domains involved in the binding of USP35 to MAVS, we constructed a series of truncations of MAVS."
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"Co-immunoprecipitation (Co-IP) experiments in 293 T co-transfected with Myc-USP35 and Flag-tagged RIG-I, MAVS, or TBK1 showed that USP35 strongly interacted with MAVS (Fig. xref )."
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"Reciprocal Co-IP assay displayed that endogenous USP35 could bind to endogenous MAVS (Fig. xref )."
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sparser
"To validate whether USP35 directly interacts with MAVS, we performed a GST Pull-down assay using the purified USP35 protein and detected a direct interaction between GST-USP35 and MAVS (Fig. xref )."
40016186
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"To explore whether the binding of USP35 to MAVS is regulated by VSV virus stimulation, we infected cells with the VSV virus."
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"Co-IP experiments showed that the transmembrane domains of MAVS interacted with USP35 (Fig. xref )."
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