IndraLab

Statements

CALM1 binds PreIQ. 5 / 5
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"Pull-down assays demonstrated that CaM1 was markedly bound to the NT, CT1, CT1B, PreIQ, and IQ peptides, whereas its binding to CT2, CT3, and CT1A was negligible (Fig. 5a-d)."
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"CaM1 binding to PreIQ and NT peptides tended to be shifted rightward by scoparone under 1 mM Ca (Fig. S13)."
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"Although CaM1 binding to the NT region and PreIQ tended to be decreased, while binding to CT1 and CT1B tended to be only slightly increased, CaM1 binding to the IQ domain was markedly increased in the presence of scoparone (47.06%, Fig. 6a-b)."
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"However, CaM1 binding to the PreIQ and NT peptides was not markedly affected by scoparone at total CaM1 concentrations greater than 1 μM under 1 mM Ca (Fig. S13)."
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"It was difficult to examine the effect of scoparone on the binding of the PreIQ and NT motifs with CaM1 under 100 nM Ca by pull-down assay because of low affinity between PreIQ and CaM1 and between the NT motif and CaM1."
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