IndraLab

Statements

ENO1 binds UCHL3. 12 / 12
2 | 10
biogrid
No evidence text available
30559450
sparser
"UCHL3 interacts with ENO1."
41271634
sparser
"These results suggest that UCHL3 interacts with ENO1 and stabilizes the ENO1 protein through deubiquitination, and that K256 and K420 are critical sites for ubiquitination and the stability of ENO1."
40487654
sparser
"Thus, molecular docking was performed to further explore the interaction between UCHL3 and ENO1, revealing a strong interaction with a binding energy of -647.804 kcal/mol (Fig. xref , enlarged figure shown in Supplementary Fig. xref )."
41271634
sparser
"To clinically validate the UCHL3-ENO1 relationship, we performed ENO1 immunohistochemistry in our cohort of 127 GC tissues."
41271634
biogrid
No evidence text available
26344197
sparser
"Furthermore, we confirmed the interaction between UCHL3 and ENO1 by transfecting Flag-UCHL3 and HA-ENO1 plasmids into 293 T cells and performing exogenous Co-IP experiments, which demonstrated their interaction (Fig. xref )."
41271634
sparser
"UCHL3 interacts with ENO1 and stabilizes the ENO1 protein through deubiquitination."
40487654
sparser
"To further explore their binding regions, we constructed a series of truncated plasmids for UCHL3 and ENO1, and found that the UCHL3 (179-230 AA) fragment interacted with the ENO1 (140-434 AA) fragment through Co-IP assays (Fig. xref )."
41271634
sparser
"Building on our findings of a strong interaction between UCHL3 and ENO1, we conducted further experiments to elucidate how UCHL3 affects ENO1."
41271634
sparser
"Our results suggest that the aberrant activation of UCHL3–ENO1 axis may be a key driver of elevated glycolysis, enhanced stem-like properties, and 5-FU chemoresistance in TP53 -mutant CRC."
40487654
sparser
"Subsequently, the interaction between UCHL3 and ENO1 proteins in HCT116 cells with wild-type TP53 and SW480 cells with TP53 mutation was confirmed through Co-IP assays ( xref B)."
40487654