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"We transiently transfected the USP46-Flag plasmids into HEK293T cells, and then performed LC-MS/MS analysis to investigate the whole proteins that could be co-immunoprecipitated with USP46 ( Fig. 5 A)[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"USP46-Flag plasmids or empty vectors were transfected into HEK293T cells for 24 h respectively and then we performed the co-immunoprecipitation assay."