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"Blockade of ENaCs by amiloride induces c-Fos activation of the area postrema."

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"Blockade of ENaCs by Amiloride Induces c-Fos Activation of the Area Postrema."

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"The c-fos cells evoked by NaCl stimulations were observed in the central medial and the external lateral subnuclei in the PBN, and the c-fos cells in the central medial were reduced by amiloride (Tokita, Shimura, Nakamura, Inoue, & Yamamoto,2007; Yamamoto etal.,1993)."

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"By contrast, c-fos induction, quantified by slit blot hybridization of c-fos mRNA, was not fully inhibited by the reduction of [Na+] o. Furthermore, amiloride and ethylisopropyl-amiloride, inhibitors of Na +/H+ exchange, only partially blocked c-fos induction by serum."

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"Amiloride and bumetanide, when added separately or in combination, did not inhibit the induction of c-fos, c-myc, and ODC mRNAs."

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"We reasoned that if amiloride blocked the c-Fos activation that occurs after hypertonic saline, these data would support the hypothesis that ENaCs localized in the CVOs may play a role in the brain sodium sensing system."