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OTUB1 binds CTNNB1. 25 / 25
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"The relationship between OTUB1 and the wnt/β-catenin signaling pathway remains unclear, previous studies indicated that OTUB1 binds with β-catenin and regulates its degradation in colorectal cancer."
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"Besides, we generally detected the interaction between OTUB1 and β-catenin by cell line models."
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sparser
"Membranous and cytoplasmic β-catenin expression was not associated with OTUB1 expression (Figure  xref B (iii) and (iv))."
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"OTUB1 interacts with β-catenin and regulates its stability."
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sparser
"The relationship between OTUB1 and the wnt/β-catenin signaling pathway remains unclear, previous studies indicated that OTUB1 binds with β-catenin and regulates its degradation in colorectal cancer."
39113709
sparser
"To further explore the specific relationship between OTUB1 and β-catenin, the network association involved in OTUB1 and β-catenin was predicted via GENEMANIA online database, and the results showed that molecular and functional interaction between OTUB1 and β-catenin was undoubtedly ( xref B)."
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sparser
"Although the relationship between OTUB1 and β-catenin has been introduced in colorectal cancer xref , our study further explained the more detailed interaction between OTUB1 and β-catenin, emphasizing the effects of the OTUB1/β-catenin axis in BLCA and the specific domain of interaction."
39113709
sparser
"A co-immunoprecipitation experiment was performed to further determine the specific relationship between OTUB1 and β-catenin, and the results showed that OTUB1 binds with β-catenin (Fig. xref I)."
39113709
sparser
"The fact that OTUB1 binds with β-catenin has been identified, and the results of immunofluorescence showed that elevated OTUB1 promotes β-catenin expression and its nuclear import, which further regulates the complicated transcription processes (Fig. xref A)."
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sparser
"The results demonstrated that OTUB1 binds with β-catenin via the canonical manner depending on the deubiquitinase catalytic activity."
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"The results further demonstrated that the C91 amino acid site plays a core role in the interaction between OTUB1 and β-catenin."
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"To explore the mechanism of the OTUB1/β-catenin axis in T24 resistance cells, the immunoprecipitation assay between OTUB1 and β-catenin was conducted, and the results further confirmed the hypothesis that OTUB1 binds with β-catenin to facilitate BLCA progression and cisplatin resistance (Fig. xref F)."
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sparser
"As we provided novel clues that OTUB1- β-catenin interaction resulted in CRC progression in this study, such interaction might enlighten new therapeutic target for CRC treatment strategy development in future."
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sparser
"Besides, we generally detected the interaction between OTUB1 and β-catenin by cell line models."
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"In this study, we further explore a deeper association between OTUB1 and β-catenin, and the role of the OTUB1/β-catenin axis on carcinogenesis and progression of BLCA, which will be a more complete supplement for OTUB1 complicated functions."
39113709
reach
"To further explore the specific relationship between OTUB1 and β-catenin, the network association involved in OTUB1 and β-catenin was predicted via GENEMANIA online database, and the results showed that molecular and functional interaction between OTUB1 and β-catenin was undoubtedly (Fig. S3B)."
39113709
reach
"A co-immunoprecipitation experiment was performed to further determine the specific relationship between OTUB1 and β-catenin, and the results showed that OTUB1 binds with β-catenin (Fig. 4I)."
39113709
reach
"The fact that OTUB1 binds with β-catenin has been identified, and the results of immunofluorescence showed that elevated OTUB1 promotes β-catenin expression and its nuclear import, which further regulates the complicated transcription processes (Fig. 5A)."
39113709
reach
"The results demonstrated that OTUB1 binds with β-catenin via the canonical manner depending on the deubiquitinase catalytic activity."
39113709
reach
"The results further demonstrated that the C91 amino acid site plays a core role in the interaction between OTUB1 and β-catenin."
39113709
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"The phosphorylation of serine33 of β-catenin has been known to be involved in the degradation, we further found that serine33 mutation cannot bind with OTUB1 in subsequent experiments (Fig. 5L) Based above results, we identified that OTUB1 (via 47-271 aa) binds with β-catenin (via 1-694 aa) and restrains its ubiquitination degradation; C91 in OTUB1 and Ser33 in β-catenin were the critical sites for the interaction and physiological function."
39113709
reach
"To explore the mechanism of the OTUB1/β-catenin axis in T24 cells, the immunoprecipitation assay between OTUB1 and β-catenin was conducted, and the results further confirmed the hypothesis that OTUB1 binds with β-catenin to facilitate BLCA progression and cisplatin resistance (Fig. 7F)."
39113709
reach
"Although the relationship between OTUB1 and β-catenin has been introduced in colorectal cancer17, our study further explained the more detailed interaction between OTUB1 and β-catenin, emphasizing the effects of the OTUB1/β-catenin axis in BLCA and the specific domain of interaction."
39113709
biogrid
No evidence text available
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biogrid
No evidence text available
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