IndraLab

Statements

USP47 binds fbxw1. 7 / 7
| 5 2
sparser
"These data showed that USP47 interacts specifically with β-Trcp."
19966869
sparser
"Unexpectedly, the USP47 (S914/918A) mutant bound endogenous β-Trcp with the same efficiency as the wild-type protein ( xref , lanes 2 and 3)."
19966869
reach
"To confirm the specific binding between USP47 and beta-Trcp, we screened 19 human F-box proteins."
19966869
reach
"These data showed that USP47 interacts specifically with beta-Trcp."
19966869
reach
"To analyze whether the binding between beta-Trcp and USP47 requires the WD40-repeat domain and an intact Arg434, we performed co-immunoprecipitation experiments using different beta Trcp mutants (all FLAG tagged)."
19966869
reach
"To analyze whether the physical interaction between USP47 and beta-Trcp resulted into a proteasome dependent degradation of USP47, we first analyzed the effect of the proteasome inhibitor MG132 on USP47 protein levels."
19966869
reach
"The specificity of the binding between USP47 and beta-Trcp and the similarity of the phenotypes produced by targeting USP47 or beta-Trcp suggested to us that USP47 could be a regulator of beta-Trcp activity."
19966869