IndraLab

Statements

MYC binds USP29. 5 / 5
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"To validate the mass spectrometry data, we performed reciprocal immunoprecipitation with co-expression of Flag-AURKB and Myc-USP29 in 293T cells and confirmed associations between these both proteins (Fig.  xref C)."
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"HEK 293 T cells were transfected with both Myc-USP29 (wild-type or with C223S mutation), HA-Ub, and Flag-FTO to confirm that USP7 controls the ubiquitination of FTO."
37605223
biogrid
No evidence text available
34601505
sparser
"To examine which kind of polyubiquitin chains USP29 removes from AURKB, we co-transfected 293T cells with Flag-AURKB, Myc-USP29 and HA-tagged ubiquitin mutants retaining a single lysine residue, and uncovered that USP29 specifically deconjugated the K48-linked polyubiquitin chains (Fig.  xref G), a well-established destruction signal to trigger 26 S proteasome-mediated proteolysis [ xref ]."
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sparser
"To further explore this notion, Flag-AURKB, Myc-USP29, HA-tagged ubiquitin (WT) or K48R mutant were co-transfected into 293T cells, and the results showed that USP29 failed to catalyze removal of K48R-linked polyubiquitin chains from AURKB (Fig.  xref H)."
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