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OTUD4 activates MHV68. 5 / 5
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"Notably, the DUB-deficient mutants (C45A and ΔDUB) also effectively rescued MHV68 replication (Fig 4E), indicating that OTUD4 promotes MHV68 lytic replication independently of its DUB activity.To investigate OTUD4’s role in MHV68 replication in vivo, we administered tamoxifen to Otud4 Cre-ERT2 and Otud4 Cre-ERT2 mice to induce OTUD4 knockout, followed by MHV68 infection via intranasal inoculation."
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"Our data indicate that knockout of OTUD4 in mice greatly impairs the replication of MHV68, a murine herpesvirus closely related to KSHV, thereby validating the critical role of OTUD4 in MHV68 replication in vivo."
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"OTUD4 deficiency impairs MHV68 replication."
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"Next, we asked whether OTUD4 also promotes the replication of MHV68, a murine herpesvirus that is genetically and biologically relevant to KSHV."
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"Knockdown of OTUD4 in NIH3T3 cells significantly impaired MHV68 lytic replication, as indicated by the decreased GFP intensity (S4A and S4B Fig)."
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