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USP39 phosphorylates syd-2. 8 / 8
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reach
"Using phosphoproteomics, we find SAD-1 phosphorylates SYD-2 on 3 sites that are critical to activate phase separation."
38048304
reach
"We conclude that SAD-1 phosphorylates SYD-2 at developing synapses, activating its phase separation and active zone assembly."
38048304
reach
"Fortuitously, a SYD-2 phosphopeptide was a top hit present in all 3 biological replicates (Fig 3A), indicating SAD-1 may be responsible for SYD-2 phosphoregulation.To test if SAD-1 was directly responsible for phosphorylating SYD-2 and determine if additional sites may be present that were not found in the phosphoproteomics screen, we performed in vitro kinase assays between SAD-1 and SYD-2 (Figs 3B, S4A, and S4B and S2 Table)."
38048304
reach
"Our data suggest SYD-2 phosphorylation by SAD-1 activates the protein to phase separate and assemble the presynaptic active zone."
38048304
reach
"We determine SAD-1 phosphorylation of SYD-2 at three sites is critical to activate its phase separation."
37398223
reach
"We conclude that SAD-1 phosphorylates SYD-2 at developing synapses, enabling its phase separation and active zone assembly."
37398223
reach
"Fortuitously, a SYD-2 phosphopeptide was a top hit present in all 3 biological replicates (Figure 3A), indicating SAD-1 may be responsible for SYD-2 phosphoregulation.To test if SAD-1 was directly responsible for phosphorylating SYD-2 and determine if additional sites may be present not found in the phosphoproteomics screen, we performed in vitro kinase assays between SAD-1 and SYD-2 (Figure 3B and S3A-B)."
37398223
reach
"Our data suggest SYD-2 phosphorylation by SAD-1 activates the protein to phase separate and assemble the presynaptic active zone."
37398223