IndraLab

Statements

USP3 binds Flag. 4 / 4
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"Similarly, in HEK293 cells, we observed protein stabilization of ectopically expressed Myc-Oct4 as the concentration of Flag-USP3 increased ( xref E), but expression of the catalytic mutant Flag-USP3CS had no such stabilization effect ( xref F)."
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"We co-transfected Flag-USP3 and Myc-Oct4 into HEK293 cells and performed IP with Flag or Myc antibodies, followed by Western blot with reciprocal antibodies."
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"To determine differentially expressed proteins that may serve as potential interaction targets of USP3, total protein extracted from the treated BGC-823 cells stably expressing the Flag-USP3 fusion protein and the BGC-823 cells carrying an empty vector was analyzed using iTRAQ experiments."
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"Flag-USP3 co-precipitated Myc-Oct4 ( xref A), and similarly Myc-Oct4 co-precipitated Flag-USP3 ( xref B)."
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