IndraLab

Statements

KAT2B binds USP22. 5 / 8
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"We observed similar results using the PCAF HAT inhibitor garcinol ( xref ), which blocked H2BK120 acetylation in vitro () while not affecting PCAF-USP22 interactions or recruitment of USP22 to DNA damage sites in treated cells ()."
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"Regardless, our data have identified and mapped interactions between PCAF and USP22, as well as further revealing the importance of the HAT and BRD domains of PCAF in mediating DNA damage signaling and repair of DSBs by HR."
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"Streptavidin pull-down of SFB-tagged PCAF validated our AP-MS, as interactions between PCAF and the SAGA DUB components USP22, ENY2, and ATXN7L were observed (Fig. 4B)."
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"In addition to PARP inhibitor sensitivity, GSK4027 treatment also reduced recruitment of PCAF and USP22 to DNA damage sites, again consistent with the BRD of PCAF being an essential domain linking PCAF to its DNA damage functions (Fig. 4I)."
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"Regardless, our data have identified and mapped interactions between PCAF and USP22, as well as further revealing the importance of the HAT and BRD domains of PCAF in mediating DNA damage signaling and repair of DSBs by HR.The observation of a H2BK120 ubiquitin to acetyl switch at DSBs (Clouaire et al. 2018) and the potential involvement of PCAF in mediating these events prompted us to test directly the involvement of PCAF in regulating H2BK120 acetylation."
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