IndraLab

Statements

POLDIP3 phosphorylates EIF3F. 16 / 16
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"This data is consistent with our previous in vitro kinase assay data and endogenous serine phosphorylation data that eIF3f is phosphorylated by CDK11 p46 after apoptotic stimulation, especially around 36 h [ xref ]."
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"The biological consequence of the phosphorylation of eIF3f by CDK11 p46 during apoptosis is not known."
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"We demonstrated that eIF3f is directly phosphorylated by CDK11 p46 in vivo ."
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"To analyze whether endogenous eIF3f is directly phosphorylated by CDK11 p46 , A375 cells were transfected with CDK11 p46 ."
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"This result strongly suggests that eIF3f is directly phosphorylated by CDK11 p46 in living cells."
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"We next investigated the effect of eIF3f phosphorylation by CDK11 p46 on its association with the eIF3 core complex during apoptosis."
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"EIF3f can be phosphorylated by CDK11 p46 during apoptosis [ xref ]."
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"To further confirm that the increased association of eIF3f with the eIF3 core complex during apoptosis is related to the phosphorylation of eIF3f by CDK11 p46 , we performed a similar experiment using CDK11 p46 transfected cells."
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"We have also presented data to show the direct phosphorylation of eIF3f by CDK11 p46 endogenously which confirmed our previous observations."
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"We identified another phosphorylation site (Thr119) in eIF3f that is phosphorylated by CDK11 p46 during apoptosis."
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"To further confirm the endogenous phosphorylation of eIF3f by CDK11 p46 during apoptosis, A375 cells were treated with anti-Fas and/or caspase 3 inhibitor (DEVD-FMK). [CDK11 p46 was produced in anti-Fas treated cells, whereas caspase 3 inhibitor blocked the production of CDK11 p46 ( xref )."
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"Endogenous phosphorylation of eIF3f by CDK11 p46 ."
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"We previously showed that eIF3f is phosphorylated by CDK11 p46 which is an important effector in apoptosis."
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"To investigate if it is the only phosphorylation site in eIF3f that is phosphorylated by CDK11 p46 , Ser46 of eIF3f was mutated to alanine."
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"Here, we identified a second site in eIF3f that is phosphorylated by CDK11 p46 during apoptosis."
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"CDK11 p46 phosphorylates eIF3f at more than one site during apoptosis."
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