IndraLab

Statements

POLDIP3 phosphorylates EIF3F. 17 / 17
| 17
sparser
"EIF3f can be phosphorylated by CDK11 p46 during apoptosis [ xref ]."
18381585
sparser
"Endogenous phosphorylation of eIF3f by CDK11 p46 ."
19245811
sparser
"We demonstrated that eIF3f is directly phosphorylated by CDK11 p46 in vivo ."
19245811
sparser
"To further confirm the endogenous phosphorylation of eIF3f by CDK11 p46 during apoptosis, A375 cells were treated with anti-Fas and/or caspase 3 inhibitor (DEVD-FMK). [CDK11 p46 was produced in anti-Fas treated cells, whereas caspase 3 inhibitor blocked the production of CDK11 p46 ( xref )."
19245811
sparser
"To further confirm that the increased association of eIF3f with the eIF3 core complex during apoptosis is related to the phosphorylation of eIF3f by CDK11 p46 , we performed a similar experiment using CDK11 p46 transfected cells."
19245811
sparser
"To analyze whether endogenous eIF3f is directly phosphorylated by CDK11 p46 , A375 cells were transfected with CDK11 p46 ."
19245811
sparser
"We identified another phosphorylation site (Thr119) in eIF3f that is phosphorylated by CDK11 p46 during apoptosis."
19245811
sparser
"The biological consequence of the phosphorylation of eIF3f by CDK11 p46 during apoptosis is not known."
19245811
sparser
"Here, we identified a second site in eIF3f that is phosphorylated by CDK11 p46 during apoptosis."
19245811
sparser
"We have also presented data to show the direct phosphorylation of eIF3f by CDK11 p46 endogenously which confirmed our previous observations."
19245811
sparser
"We next investigated the effect of eIF3f phosphorylation by CDK11 p46 on its association with the eIF3 core complex during apoptosis."
19245811
sparser
"CDK11 p46 phosphorylates eIF3f at more than one site during apoptosis."
19245811
sparser
"To investigate if it is the only phosphorylation site in eIF3f that is phosphorylated by CDK11 p46 , Ser46 of eIF3f was mutated to alanine."
19245811
sparser
"We previously showed that eIF3f is phosphorylated by CDK11 p46 which is an important effector in apoptosis."
19245811
sparser
"This data is consistent with our previous in vitro kinase assay data and endogenous serine phosphorylation data that eIF3f is phosphorylated by CDK11 p46 after apoptotic stimulation, especially around 36 h [ xref ]."
19245811
sparser
"This result strongly suggests that eIF3f is directly phosphorylated by CDK11 p46 in living cells."
19245811
sparser
"It was recently reported that CDK11 p58 repressed cap-dependent mRNA translation by phosphorylating subunit eIF3F of the translation factor eIF3 complex [ 81 ], confirming a previous study showing tha[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32446654