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TGFB1 increases the amount of SDC4. 19 / 19
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"Together, these results suggest that the early upregulation of endothelial syndecan-4 expression by TGF-beta 1 is mediated by the ALK5-Smad3-p38 MAPK pathway, whereas the late downregulation is mediated by the ALK5-Smad2/3 pathway."
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"To examine the involvement of Smad2 and Smad3 in the modulation of syndecan-4 expression by TGF-beta 1, vascular endothelial cells were transfected with siSmad2 or siSmad3 and then treated with 5ng/mL TGF-beta 1."
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"These results indicate that the early upregulation of syndecan-4 mRNA expression by TGF-beta 1 is mediated by Smad3, whereas Smad2 or Smad3 alone is not involved in the late downregulation of syndecan-4 mRNA expression by TGF-beta 1; the late downregulation of syndecan-4 core protein expression by TGF-beta 1 is mediated by Smad3."
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"The early upregulation and late downregulation of syndecan-4 mRNA expression by TGF-beta 1 were both not affected by siRNA mediated knockdown of Smad2."
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"The results indicated that the siRNA mediated knockdown of both Smad2 and Smad3 diminished the late downregulation of syndecan-4 mRNA expression by TGF-beta 1, although the knockdown of just one could not diminish the downregulation."
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"These results and our data, which showed that the induction of endothelial syndecan-4 expression occurs in the early stage of the exposure to TGF-beta 1, suggest that the upregulation of endothelial syndecan-4 expression by TGF-beta 1 may be a defense mechanism against an acute injury to vascular endothelial cells."
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"These results suggest that the late downregulation of syndecan-4 expression by TGF-beta 1 is mediated by Smad2/3 at the mRNA level and by Smad3 at the protein level."
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"Specifically, the downregulation of syndecan-4 expression by TGF-beta 1 is mediated by the interaction of Smad3 with Smad2 at the mRNA expression level, whereas only Smad3 is crucial at the core protein expression level."
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"It is possible that the biphasic regulation of endothelial syndecan-4 expression by TGF-beta 1 is mediated by the Smad pathway, non Smad pathway, or both."
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"THE p38 MAPK PATHWAY MEDIATES THE EARLY UPREGULATION OF ENDOTHELIAL SYNDECAN-4 EXPRESSION BY TGF-beta 1."
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"Furthermore, SDC4 overexpression enhanced the induction of EMT related genes by the stimulation of TGF-beta1 in the both A549 and NCI-H292 cells."
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"Figure XREF_FIG shows the phosphorylation of MAPKs (ERK1/2, JNK, and p38 MAPK) and Smad2/3, which may be involved in the modulation of syndecan-4 expression by TGF-beta 1 as the downstream signaling pathways of the cytokine."
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"On the other hand, siRNA mediated knockdown of Smad3 diminished the upregulation of syndecan-4 mRNA expression by TGF-beta 1, suggesting that Smad3 is a member of the signaling pathway that mediates the TGF-beta 1 -induced upregulation of syndecan-4 expression."
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"This result is consistent with our recent study [Hara et al., 2016], showing that TGF-beta 1 modulates endothelial syndecan-4 expression in a biphasic manner."
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"TGF-beta1 up-regulated the expression of SDC4, accompanied by the induction of EMT."
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"In addition, TGF-beta 1 increased syndecan-4 core protein expression in the cell layer, and this increase was completely diminished by SB203580; the core protein was not detected in the conditioned medium."
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"To examine the involvement of MAPKs in the modulation of syndecan-4 mRNA expression by TGF-beta 1, vascular endothelial cells were pretreated with the MEK1 inhibitor PD98059, JNK inhibitor SP600125, or p38 MAPK inhibitor SB203580 and then stimulated with TGF-beta 1."
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"The late downregulation of syndecan-4 core protein expression by TGF-beta 1 was observed in cells treated with or without siRNA for Smad2, but this downregulation was reduced by siRNA for Smad3."
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"They indicated that TGF-beta 1 upregulates syndecan-4 expression via the ALK5-Smad3-p38 MAPK pathway at the early stage, whereas downregulation of the expression occurs via the ALK5-Smad2/3 pathway."
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