IndraLab

"This interaction was associated with the RTK/RAS pathway and may serve as a potential marker for poor clinical outcomes. xref In O, we observed a variety of ligand-receptor pairs, including TNFRSF13B-TNFSF13 , TNFRSF13B-CD70 , TIGIT-NECTIN2 , NTRK1-NAMPT , FGFR1-FGF9 , EREG-EGFR , and CXCL1-ACKR1 ( xref F and xref B), which are also correlated with the RTK/RAS pathway. xref Notably, Tregs secreted TIGIT , which binds to ENCTIN2 on endothelial cells, potentially contributing to the immunosuppressive environment. xref Monocytes secreted EREG , which binds to EGFR on endothelial cells, suggesting that targeting the EREG-EGFR axis could have therapeutic potential for inhibiting tumor progression. xref In LN, specific ligand-receptor pairs, such as VGF-NTRK1 , NOTCH1-NOV , NOTCH4-JAG2 , PVR-TIGIT , PVR-NECTIN3 , CXCR2-CXCL5 , CX3CR1-CX3CL1 , and CCR1-CCL14 , were highly expressed ( xref F and xref D)."

"For example, dynamic changes in the TIME are analyzed based on the overall expression levels of specific genes, and prognostic biomarkers have been developed to predict bladder cancer outcomes. xref However, bulk RNA-seq measures the average gene expression across the entire transcriptome of all cells, which overlooks potential cellular heterogeneity. xref Single-cell RNA sequencing (scRNA-seq) is a revolutionary genomics technology that enables comprehensive analysis of gene expression profiles at the single-cell level, revealing the roles of different cell subsets and intercellular communication within tumors. xref For example, Meng et al found that tumor cells can reprogram CD10+ALPL+ neutrophils through the NAMPT-NTRK1 signaling axis, leading to immune resistance and HCC progression. xref Therefore, it is necessary to develop biomarkers that guide clinical decision-making and provide personalized therapy by integrating scRNA-seq and bulk RNA-seq."

"Recent research advancements have unveiled intricate molecular mechanisms underlying neutrophil “betrayal”, including activation by tumor cells via various signaling pathways, such as the NAMPT-NTRK1 signaling axis, and the expression of immunosuppressive cytokines like IL-10 and TGF-β [ xref , xref ]."