IndraLab

Statements

USP8 binds STAM. 34 / 35
1 5 1 | 17 10
reach
"USP8 can form complexes with the ESCRT-0 subunit STAM on endosomes, stimulating de-ubiquitination of EGFR on endosomes, and promoting EGFR endosome-to-plasma membrane recycling [XREF_BIBR]."
29543760
sparser
"Hence, AMSH knockdown could conceivably enhance UBPY binding to STAM, or VPS4 binding to ESCRT-III components ( Figure 4 )."
16996268
sparser
"It has been shown previously that a subset of SH3 domains bind ubiquitin ( xref , xref ), and a recent study using NMR titration experiments showed that the SH3 domain of STAM does in fact bind ubiquitin, and that this interaction can be competed off by USP8 binding to the SH3 domain of STAM ( xref )."
24151880
sparser
"However, it remained unclear whether the USP8–STAM complex interacts with Sec31A. Our data showed that Sec31A interacted specifically with the USP8–STAM1 complex, but not with the USP8–STAM2 complex."
29604273
sparser
"The USP8–STAM1 complex may constantly interact with Sec31A and deubiquitinate Sec31A immediately after its ubiquitination, thereby tightly regulating Sec31A ubiquitination levels."
29604273
signor
"Stability of the UBPY binding partner STAM is dramatically compromised in UBPY knockdown cells."
16520378
biogrid
No evidence text available
20736164
biogrid
No evidence text available
10982817
biogrid
No evidence text available
20736164
reach
"Hrs is constitutively associated with STAM, which in turn can bind to the deubiquitinating enzyme UBPY [38], implying that the correlation between E3 ligase POSH and UBPY (or unidentified DUB) might m[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16084064
sparser
"The physiological roles of the USP8–STAM1 complex in the regulation of collagen secretion are still unclear."
29604273
sparser
"Further studies should examine roles of the USP8–STAM1 complex in the transport of these macromolecules."
29604273
sparser
"Finally, we anticipate that our future work will propose the USP8–STAM1 complex as a novel therapeutic target in collagen-related diseases or other diseases caused by aberrant function of COPII-relate[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
29604273
reach
"By binding to STAM and Hrs, USP8 stabilizes the ESCRT-0 complex, which is the crucial element directing ubiquitinated substrates toward MVB/lysosome-mediated degradation (Mizuno et al., 2006; Row et al., 2006)."
39576689
reach
"Both AMSH and Usp8 bind to ESCRT-0 protein STAM through a non canonical SH3 binding motif, and with their MIT-domain to several CHMP proteins of the ESCRT-III machinery [30,37,39,40]."
22800866
reach
"STAM also interacts with and recruits USP8 to the endosome, resulting in the deubiquitination of EGFR [ 16 ]."
29604273
reach
"These results demonstrated that the USP8–STAM1 complex deubiquitinates Sec31A.Next, we tested whether USP8 regulates COPII carrier size."
29604273
reach
"However, it remained unclear whether the USP8–STAM complex interacts with Sec31A."
29604273
reach
"Our data showed that Sec31A interacted specifically with the USP8–STAM1 complex, but not with the USP8–STAM2 complex."
29604273
sparser
"That STAMs could be components of a larger complex of a ubiquitin machinery is suggested by a study of Kato et al. [31] , showing that the deubiquitinating enzyme UBPY binds to the SH3 domain of STAM[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11728436
reach
"The USP8–STAM1 complex may constantly interact with Sec31A and deubiquitinate Sec31A immediately after its ubiquitination, thereby tightly regulating Sec31A ubiquitination levels."
29604273
reach
"In contrast, stability of the UBPY binding partner STAM is dramatically compromised in UBPY knockdown cells."
16520378
biogrid
No evidence text available
29604273
reach
"Because the structure of large COPII carriers varies by experimental conditions ( Fig. 3 and supplementary Fig. 1 ), careful attention should be given to the conditions in further mechanistic analyses[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
29604273
reach
"Further studies should examine roles of the USP8–STAM1 complex in the transport of these macromolecules."
29604273
reach
"Finally, we anticipate that our future work will propose the USP8–STAM1 complex as a novel therapeutic target in collagen-related diseases or other diseases caused by aberrant function of COPII-relate[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
29604273
reach
"STAM also directly interacts with two endosomal DUBs, ubiquitin-specific protease Y [UBPY; also known as ubiquitin-specific processing protease (USP) 8] and associated molecule with the Src homology 3[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16996268
reach
"Hence, AMSH knockdown could conceivably enhance UBPY binding to STAM, or VPS4 binding to ESCRT-III components ( Figure 4 )."
16996268
biogrid
No evidence text available
16520378
sparser
"Hrs is constitutively associated with STAM, which in turn can bind to the deubiquitinating enzyme UBPY [38] , implying that the correlation between E3 ligase POSH and UBPY (or unidentified DUB) might[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16084064
hprd
No evidence text available
10982817
reach
"The Adenosine A2 receptor is deubiquitinated by USP4; the ubiquitination status and trafficking of the EGFR growth factor receptor is regulated by the USP8 and STAM complex; the beta2-AR undergoes increased agonist stimulated ubiquitination, lysosomal trafficking, and degradation after knockdown of USPs 20 and 33."
21898592
reach
"Similar overexpression of UIM-domain mutants of Hrs does not exert this negative effect on vesicle formation, implying that release of Hrs from bound cargo proteins might provide a control mechanism t[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
14624836
sparser
"These results demonstrated that the USP8–STAM1 complex deubiquitinates Sec31A. Next, we tested whether USP8 regulates COPII carrier size."
29604273