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SNAI1 binds EIF3H. 13 / 13
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"Next, immunoprecipitation of endogenous Snail from KYSE510 cells also confirmed the interaction between endogenous Snail and EIF3H."
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"EIF3H interacts with Snail."
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"To shed light on how EIF3H interacts with Snail, we further determined their cellular localization by immunofluorescence analysis."
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"Our study reveals a critical EIF3H-Snail signaling axis in tumor aggressiveness in ESCC and provides EIF3H as a promising biomarker for ESCC treatment."
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"We performed a co-IP assay combined with the mass spectrometry analysis and identified that an EMT associated transcription factor, Snail could interact with EIF3H."
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"In order to get a comprehensive understanding about the significance of EIF3H and the mechanism of its function in ESCC, we performed a liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis and identified that EIF3H could interact with Snail and correlate positively with Snail expression."
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"We demonstrated that EIF3H interacts with and stabilizes Snail through deubiquitination."
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"Additional in vivo co-IP assay was performed in HEK293T and KYSE510 EIF3H-knockdown cells to test the specificity of the interaction between Snail and EIF3H."
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"EIF3H interacts with and deubiquitinates Snail to prolong its half-life."
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"The interaction between EIF3H and Snail suggests the ability of EIF3H to regulate Snail expression."
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"To confirm the interaction between EIF3H and Snail, we then performed a co-IP assay with co-expressed EIF3H-Flag and Snail in HEK293T cells."
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"In vivo co-IP assays in EIF3H knockdown cells were also carried out to confirm the specificity of the interaction between Snail and EIF3H, but there is no interaction with other EIF3 subunits (EIF3A and EIF3I)."
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"These results revealed the interaction between EIF3H and Snail mediated by the S-P rich domain of Snail."
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