IndraLab
Statements
reach
"Regarding the upstream components of the pathway, the sources of significant variance detected by two-way ANOVA tests were due to similarly elevated levels of insulin receptors (Figure 5A), IGF-1 receptors (Figure 5D), and p/T IRS-1 (Figure 5I), and reduced expression of pY-Insulin receptors (Figure 5B), p/T Insulin (Figure 5C) and IGF-1 (Figure 5F) receptors, and IRS-1 (Figure 5G) in CS and ES relative to CC and/or EC."
reach
"Insulin resistance was induced by palmitic acid (PA) in human HK-2 cells, shown as the decrease of insulin stimulated AKT phosphorylation, glucose transporter-4 (GLUT4), glucose uptake and enhanced phosphorylation of insulin receptor substrate 1 (IRS-1) at site serine 307 (pIRS-1ser307) and downregulated expression of IRS-1."
reach
"The present data revealed that insulin + GJG treatment inhibits the increased IRS-1 tyrosine phosphorylation levels (XREF_FIG); therefore, these improvements are thought to improve the abnormal activation of PI 3-kinase resulting in the correction of decreased efficiency of glucose metabolization."
reach
"In our previous study, we observed that 10 M insulin significantly promoted INSR phosphorylation and IRS1 protein expression at 2 h in alveolar bone marrow MSCs, and obviously inhibited INSR phosphorylation and IRS1 protein expression by day 7, despite this, the treatment particularly enhanced cell proliferation and osteogenic differentiation [48]."
reach
"The results showed that 100nM insulin could induce SOCS3 mRNA expression but not protein expression, and overexpression of SOCS3 decreased IRS1 protein level, insulin stimulated IRS1 tyrosine phosphorylation, PI3K activation, and Akt phosphorylation, but increased IRS1 serine phosphorylation in porcine primary adipocytes."
reach
"In contrast, Chen et al. [13] have shown that Ucn2 knockout animals were more sensitive to insulin when compared to wild animals and in vitro studies [13,80] have reported that Ucn2 decreased the phosphorylation levels of Akt (Ser ), IRS-1 (Tyr ), and ERK1/2 (Thr /Tyr ) in differentiated muscle cells incubated with insulin."
reach
"Insulin decreased mRNA expression of IRS-1 by 72% (from 0.75 +/- 0.06 to 0.21 +/- 0.04 relative units; P < 0.001), IRS-2 by 71% (from 0.55 +/- 0.10 to 0.16 +/- 0.08 relative units; P < 0.03), and Shc by 25% (from 0.95 +/- 0.04 to 0.71 +/- 0.04 relative units; P < 0.01) vs. baseline as demonstrated in the control subjects."
reach
"In contrast, whereas in group E2 insulin resistance throughout the hormonal treatment was related to diminished expression and phosphorylation of IRS-1, in group E the decrease in insulin sensitivity between days 11 and 16 of treatment was not related to a decrease in IRS-1 expression."
reach
"Insulin stimulated tyrosine phosphorylation of the insulin receptor beta-subunit and insulin receptor substrate-1 (IRS-1) in intact skeletal muscle of SHROB was reduced by 36 and 23%, respectively, compared with SHR, due primarily to 32 and 60% decreases in insulin receptor and IRS-1 protein expression, respectively."
reach
"136 A linear regression analysis has suggested that TSH is also independently and positively associated with HOMA-IR.137 Moreover, recent in vitro studies in mice have reported that TSH stimulates nuclear factor-kappa B (NF-κB) DNA-binding activity via the CAMP-PKA-dependent pathway to increase TNF-α transcriptional activity.138,139 The possible mechanisms by which TNF-α impairs insulin signal transduction include downregulating the expression of IRS-1, inhibiting tyrosine phosphorylation of IRS-1, and increasing serine/threonine phosphorylation of IRS-1."