IndraLab

Statements

USP18 binds WT1. 10 / 10
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"By luciferase reporter assay we identified the shortest promoter fragment responsible for WT1-mediated repression and also demonstrated direct binding of WT1 to the promoter of Usp18 ."
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"In order to study further the interaction between WT1 and the Usp18 promoter, we attempted to identify regions in the promoter conserved between mouse and human."
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"While we were able to demonstrate robust binding of WT1 to the Usp18 promoter by ChIP-Seq, this promoter was not identified by a ChIP-chip analysis of chromatin from a later stage of mouse kidney development (E18.5) [ xref ]."
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"Furthermore, direct binding of WT1 to the Usp18 promoter was demonstrated by ChIP assay."
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"In both murine and human cell lines, an increase expression of USP18 is associated with the activity of WT1."
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"WT1 binds directly to the Usp18 promoter and suppresses its transcription."
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"Furthermore, direct binding of WT1 to the Usp18 promoter was demonstrated by ChIP assay."
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"In order to determine whether WT1 binds to the Usp18 promoter, cross-linked protein-DNA fragments were immunoprecipitated by anti-WT1 antibody from M15 cell lysates."
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"Furthermore, to demonstrate WT1 binding to the USP18 promoter in human cells, we used T-REx™ -293 WT1(-KTS) cells in which FLAG-tagged WT1 (-KTS) expression can be induced."
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"Collectively these results indicate that WT1 specifically binds to the endogenous Usp18 promoter."
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