IndraLab

Statements

USP19 binds FLI1 and EWSR1. 5 / 5
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"USP19 interacts with the N-terminal domain of EWS-FLI1 and full length EWSR1."
30700749
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"Most interestingly, co-expression of active USP19 with EWS-FLI1 stabilized the fusion protein whereas full length EWSR1 and FLI1 protein levels remained unchanged even though binding of USP19 to both EWS-FLI1 and full length EWSR1 was observed in immunoprecipitation experiments."
30700749
sparser
"More recently, USP19 was shown to deubiquitinate and stabilise EWS-FLI-1 fusions but not FLI-1, likely because USP19 bound to EWS-FLI-1 within N-terminal EWS sequences [ xref ]."
34066106
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"It is therefore likely that EWS-FLI1 interacts with USP19 in the cytosol as the fusion protein displays a constant turnover and is shuffling in and out of the nucleus xref ."
30700749
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"For this, we co-expressed myc-USP19 with 3xflag-EWS-FLI1 wild-type or mutants (K298R or K380R) and observed binding of USP19 to both EWS-FLI1 mutants, indicating that the interaction itself is independent of the presence of an ubiquitin chain (Fig.  xref )."
30700749