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USP48 binds ARL3. 8 / 8
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"These results were consistent and independent of the epitope tag used (Figure S4), overall suggesting that the USP48 interaction and stabilisation of ARL3 and UNC119a occur by different mechanisms.In order to dissect which USP48 domain was involved in the interaction, we performed co-immunoprecipitation experiments with either ARL3 or UNC119a and several USP48 proteins carrying different domain deletions."
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"USP48 Interacts with ARL3 and UNC119a."
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"To study whether the interaction between USP48 and ARL3 and UNC119a was dependent on the USP48 DUB activity, the protein stability of these interactors was assayed in different conditions, and several USP48-derived constructs (USP48 WT , catalytically inactive USP48 C98S or hyperactive USP48 S886−888D )."
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"ARL3 co-immunoprecipitated with all USP48-derived proteins, indicating that the interaction between USP48 and ARL3 most probably occurs via the UCH catalytic domain of USP48 ( xref A)."
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"To study whether the interaction between USP48 and ARL3 and UNC119a was dependent on the USP48 DUB activity, the protein stability of these interactors was assayed in different conditions, and several USP48-derived constructs (USP48 , catalytically inactive USP48 or hyperactive USP48 )."
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"ARL3 co-immunoprecipitated with all USP48-derived proteins, indicating that the interaction between USP48 and ARL3 most probably occurs via the UCH catalytic domain of USP48 (Figure 7A)."
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"This result suggested that the CK2 domain, and to a minor extent the UBL domain, were crucial for the interaction between UNC119a and USP48.In summary, all our data support that USP48 can interact with and stabilise ARL3 and UNC119a using different mechanisms and distinct protein domains."
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"USP48 also interacts with IRD-associated proteins ARL3 and UNC119a and stabilizes their protein levels using different mechanisms, indicating its importance in intracellular protein transport and ciliary trafficking to the photoreceptor outer segment."
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