IndraLab

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(-)-epigallocatechin 3-gallate inhibits DDX58. 19 / 19
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"EGCG inhibited poly(I∶C)-dependent signaling of RIG-I K270A mutant to the same level as the wild type ( xref )."
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"Interestingly, EGCG did not inhibit the constitutive signaling activity of RIG-I (XREF_FIG)."
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"Furthermore, EGCG dose-dependently inhibited the ATPase activity of recombinant RIG-I but did not compete with RIG-I interaction with RNA or with ATP."
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"We demonstrate that treatment with EGCG, in the absence of a viral infection, increased the mRNA expression levels of RIG-I, which appear to be in contrast to a recent study which showed that EGCG suppresses Rig-I signaling XREF_BIBR."
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"Furthermore, EGCG did not inhibit the constitutive activities of RIG-I deletion mutants R-C and R-CH (XREF_FIG)."
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"At 2 microM, EGCG, its epimer GCG, and the digallate TFDG reduced RIG-I signaling by more than 60% (XREF_FIG)."
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"However, 1 microM of EGCG inhibited the ATPase activity of RIG-I to 30% in the presence of 2 mM ATP, suggesting that EGCG does not interfere with ATP binding site or acts on portions of RIG-I in addition to the ATP binding site."
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"EGCG inhibited shR9 dependent RIG-I signaling in a concentration dependent manner, with an IC 50 between 1 to 2 microM in the cell based reporter assay (XREF_FIG)."
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"We demonstrate that treatment with EGCG, in the absence of a viral infection, increased the mRNA expression levels of RIG-I, which appear to be in contrast to a recent study which showed that EGCG suppresses Rig-I signaling [58] ."
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"EGCG inhibited RIG-I signaling in a cell based reporter assay and RNA dependent ATPase activity of recombinant RIG-I protein in biochemical assays."
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"While these studies provide several features for the interaction, additional studies will be required to elucidate of the mechanism (s) through which EGCG inhibits RIG-I signaling."
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"These results show that EGCG could inhibit RIG-I after RNA binding, although it does n't inform us as to whether RNA remains bound after EGCG binding."
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"EGCG at 2 microM reduced RIG-I signaling induced by 10 nM RNA."
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"EGCG reduced RIG-I signaling at all concentrations of the transfected RIG-I plasmid tested (XREF_FIG)."
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"Lastly, the green tea molecule EGCG has also been shown to inhibit the ATPase function of RIG-I (334)."
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"The identification of EGCG inhibition of RIG-I activity provides proof of principle for small molecule modulators of this target."
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"EGCG inhibits RIG-I signaling."
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"Increasing the concentration of recombinant RIG-I protein in the presence of constant amount of EGCG and shR9 reduced the inhibitory effect of EGCG, likely by titrating away EGCG (XREF_SUPPLEMENTARY)."
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"The time of addition experiment suggested that EGCG could inhibit RIG-I only after agonist binding and EGCG did not compete with RNA binding (XREF_FIG)."
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