IndraLab

Statements

MYC binds USP43. 17 / 17
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"To test whether c-Myc directly binds to the USP43 promoter, we performed chromatin immunoprecipitation (ChIP) and qPCR."
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"Collectively, these data suggest that c-Myc can directly bind to the promoter region of USP43 and activate USP43 transcription, confirming that c-Myc is a transcription factor for USP43."
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"Coimmunoprecipitation assays indicated that several c-Myc mutants could interact with USP43, similar to wild-type c-Myc (Supplementary Fig. xref )."
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"Next, we performed a coimmunoprecipitation assay to investigate the potential interaction between USP43 and c-Myc."
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"Additionally, the interaction between USP43 and c-Myc reduces the proximity of FBXW7 to c-Myc, indirectly stabilizing it."
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"As shown in Fig. xref , USP43 interacted with the N-, N1-, N2-, and C1-terminus of c-Myc, while c-Myc interacted with both the N-terminus and the C-terminus of USP43."
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"Moreover, endogenous USP43 interacted with c-Myc in T24 and 5637 cells (Fig. xref and Supplementary Fig. xref )."
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"These findings indicate the interaction between USP43 and c-Myc."
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"However, the efficacy of drugs targeting USP43 will be dependent on their ability to inhibit the deubiquitinase activity of USP43 and degrade USP43 protein or disrupt USP43-cMyc interaction."
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"Additionally, the interaction between USP43 and c-Myc reduces the proximity of FBXW7 to c-Myc, indirectly stabilizing it."
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"Our coimmunoprecipitation assay demonstrated that the MBI domain of c-Myc can indeed interact with USP43, and the interaction was weakened when the MBI domain was deficient in the c-Myc mutant (Fig. xref and Supplementary Fig. xref )."
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"USP43 interacts with c-Myc."
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"Next, we performed a coimmunoprecipitation assay to investigate the potential interaction between USP43 and c-Myc."
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"These findings indicate the interaction between USP43 and c-Myc."
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"Since both USP43 and FBXW7 can bind to c-Myc, we hypothesized that they might compete for binding to c-Myc.There has been evidence that FBXW7 binds to the Myc box I (MBI) of c-Myc in previous studies [31], and we wondered whether USP43 also had the ability to bind to the MBI of c-Myc."
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"Furthermore, MYC transcriptionally activates POLD1 and USP43, forming reciprocal positive feedback loops (POLD1-MYC and USP43-MYC) that promote BLCA progression [ xref , xref ]."
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"In BLCA, DNA polymerase POLD1 and the ubiquitin-specific protease USP43 competitively bind to MYC, preventing its interaction with FBXW7 and thereby stabilizing MYC protein levels."
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