IndraLab

Statements

P97 binds USP13. 5 / 5
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"The data also suggest that the interaction of USP13 with p97 is mediated by several cellular factors with gp78 being a major one."
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"Compared to the control (cells transfected with an empty vector), expression of several UBX domain-containing p97 cofactors including ASPL, SAK1, and UbxD1 reduced the p97-USP13 interaction ( xref , lanes 3, 5, 7 vs lane 1), suggesting that these factors may compete with USP13 for p97 binding."
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"By contrast, several other factors enhanced the p97-USP13 interaction."
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"Secondly, depletion of gp78 by shRNA-mediated gene knockdown or gene deletion reduced the USP13-p97 interaction by threefold to fourfold ( xref , xref )."
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"We used the GST pull-down assay to screen a collection of known p97 interactors in order to identify factor(s) that promotes the USP13-p97 interaction."
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