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USP8 binds DDX3X. 11 / 11
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"To determine whether USP8 interacts with DDX3X directly, the recombinant His-USP8 and His-GFP-DDX3X proteins were purified from Escherichia coli BL21 (DE3) cells ( Figure 3 C) followed by reciprocal c[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"We observed that USP8 was bound to DDX3X ( Figure 3 C), suggesting a direct interaction between USP8 and DDX3X. Furthermore, we truncated DDX3X into the N-terminal IDR (1–168) and the remaining C-term[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"The N- or C-terminal region of DDX3X interacted with USP8 to a similar extent as did full-length (FL) DDX3X ( Figure 3 D)."
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"In addition, endogenous USP8 interacted with endogenous DDX3X upon HSV60 stimulation in THP-1 cells ( Figure 3 E)."
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"Interestingly, we observed that USP8 and DDX3X also formed colocalized condensates with classical SG stimulators including sodium arsenite and poly(I:C) in HeLa cells ( Figure S3 C)."
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"The colocalization of USP8 and DDX3X condensates was also observed in BJ fibroblasts stimulated with HSV60, suggesting that the interaction between USP8 and DDX3X was not cell type specific ( Figure S[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"We observed that USP8 was bound to DDX3X ( Figure 3 C), suggesting a direct interaction between USP8 and DDX3X."
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"The colocalization of USP8 and DDX3X condensates was also observed in BJ fibroblasts stimulated with HSV60, suggesting that the interaction between USP8 and DDX3X was not cell type specific ( Figure S[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"We demonstrated that USP8 interacted with the SG protein DDX3X, which was directly associated with cGAS and enhanced the phase separation of cGAS."
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"Given the interaction between DDX3X and USP8, we next investigated whether ubiquitinated DDX3X was a substrate of USP8."
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"Given the interaction between DDX3X and USP8, we next investigated whether ubiquitinated DDX3X was a substrate of USP8."
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