IndraLab

Statements

OTUD7B binds TP53. 9 / 9
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"Furthermore, given both HepG2 and SMMC-7721 cells are known to express wildtype (WT) p53, it was important to determine if p53 mutations which are prevalent in HCC affect OTUD7B-p53 interactions."
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"Others have shown retinoic acid (RA) treatment increases OTUD7B levels in HCC xref while we observed that OTUD7B was subject to transcriptional repression by p53: the latter finding further suggests that p53 establishes negative feedback towards tuning the cellular levels of OTUD7B. It is further noteworthy to consider how the wiring of the OTUD7B-p53 relationship may dovetail with the well-known Mdm2-p53 regulatory circuit."
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"Clinical data analysis showed a positive correlation between OTUD7B and p53 protein levels in HCC tissues, suggesting that the OTUD7B-p53 axis may represent a potential therapeutic target for HCC xref ."
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"Thus, OTUD7B physically associates with p53 through dual interfaces within the p53 TAD (1-60) and DBD (98-292) regions.Additional characterization using nuclear-cytoplasmic separation showed a biased distribution of OTUD7B within the cytoplasm of HCC cells while p53 occurred both in the cytoplasm and nucleus (Figure 1I)."
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"Furthermore, given the observation that OTUD7B binds mutant forms of p53, it was relevant to examine the capability of OTUD7B to deubiquitinate mutant p53."
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"Moreover, interactions between the endogenous OTUD7B and p53 proteins were demonstrable in HepG2 cells (Figure xref F)."
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"Further mapping experiments were then conducted using a range of p53 deletion constructs (Figure xref G) towards defining the regions of interaction between OTUD7B and p53."
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"Thus, OTUD7B physically associates with p53 through dual interfaces within the p53 TAD (1-60) and DBD (98-292) regions."
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"Further mapping experiments were then conducted using a range of p53 deletion constructs (Figure 1G) towards defining the regions of interaction between OTUD7B and p53."
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