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USP19 activates GFP1. 5 / 5
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"Like GFP, GFP1–10 secretion was enhanced by wild-type (WT) USP19, but not by the catalytically inactive USP19 mutant or a mutant lacking the TM segment (USP19 ) (Fig. 2b,d), and it was not sensitive to BFA (Supplementary Fig. 1e)."
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"We also analysed GFP1–10-containing vesicles in cells expressing different USP19 variants: WT USP19, but not USP19 or USP19 , significantly increased the number of GFP1–10 vesicles (Fig. 8b,c)."
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"As expected, overexpression of USP19 in both USP19-deficient and control cells increased GFP1–10 secretion to a similar level (Fig. 3f)."
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"Importantly, the level of membrane association correlates with the secretion efficiency: more GFP1–10 bound to the membranes than GFP in both control and USP19-overexpressing cells, and USP19 overexpression enhanced membrane interaction for both GFP and GFP1–10."
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"Moreover, USP19 depletion consistently reduced GFP1–10 in membrane fractions (Fig. 6b)."
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