IndraLab

Statements

MYC binds USP35. 7 / 7
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"We transfected Flag-Snail1 and HA-Ub, together with Myc-USP35 or empty vector, into HEK293T and MKN-45 cells and performed a Co-IP assay."
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"We co-expressed MAVS-Flag, USP35-Myc, and HA-Ub or HA-Ub K63O (K63 only) in 293 T. The results showed that USP35 removed the K63-type ubiquitination of MAVS (Fig. xref )."
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"Next, we observed an increased levels of BRPF1 proteins when C42-B cells were transfected with elevated doses of Myc-USP35 plasmids, suggesting that USP35 promotes BRPF1 levels in a dose-dependent manner (Fig. xref )."
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"We further transfected Myc-USP35 (WT) or the USP35-C450A mutant (CA) with loss of DUB activity, together with HA-Ub and Flag-Snail1, into AGS and HEK293T cells to determine whether the DUB activity of USP35 is indispensable for the deubiquitination of Snail1."
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"Therefore, to investigate the mode of deubiquitination of Snail1 by USP35, we co-transfected Flag-Snail1 with WT HA-Ub, K0 HA-Ub (with all lysine residues in Ub were mutated to arginine), or one among HA-Ub K6, K11, K27, K29, K33, K48, and K63 (in which all lysine residues except those at positions 6, 11, 27, 29, 33, 48, and 63, respectively, were replaced with arginine), together with empty vector or Myc-USP35, into HEK293T cells."
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"Restoration of Myc-USP35 in USP35-depleted A375 inhibited TBK1 and IRF3 activation upon RNA virus stimulation (Supplementary Fig. xref )."
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"Co-immunoprecipitation (Co-IP) experiments in 293 T co-transfected with Myc-USP35 and Flag-tagged RIG-I, MAVS, or TBK1 showed that USP35 strongly interacted with MAVS (Fig. xref )."
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