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Statements

OTUB1 is phosphorylated. 52 / 52
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sparser
"However, none of these kinases phosphorylated OTUB1 in vitro ( xref )."
25872870
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"Several studies have determined that OTUB1 is phosphorylated on multiple residues ( xref , xref )."
30400005
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"To investigate the involvement of OTUB1 and Src in cathepsin K inhibitor (odanacatib; ODN)-mediated Raptor destabilization, we determined the phosphorylation of OTUB1 and Src in ODN-treated cells using a specific phospho-antibody."
37330581
sparser
"It will be of interest to understand the molecular mechanisms by which phosphorylation of the catalytic OTU domain of A20 alters linkage specificity."
27913667
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"Also linking CK2 with the proteasome, this kinase phosphorylates the deubiquitinase OTUB1, modulating its nuclear deubiquitination activity and thereby stabilizing chromatin-binding proteins ( xref )."
32850305
sparser
"The phosphorylation of OTUB1 usually results in cell location changes, which further affects the deubiquitination function of OTUB1 in most cases."
34875341
rlimsp
"Our findings uncover a signal-induced phosphorylation-dependent recruitment of OTUB1 to its target in the TGFβ pathway."
24071738
sparser
"However, the exact roles of the phosphorylation of Otubain-1 and Gαq in Yersinia infection, as well as the roles of the kinase activity of YpkA in actin cytoskeleton disruption, remain elusive [8,10,1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20493189
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"OTUD5, but not yOtu1, requires activation by phosphorylation in the OTU domain, which leads to the formation of the S1 Ub-binding site ( xref ) ( xref E)."
23827681
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"CK2-mediated phosphorylation of OTUB1 at S16 acts as a molecular switch that drives nuclear accumulation, which is essential for repairing ionizing radiation-induced DNA damage, independent of changes to its intrinsic catalytic activity. xref This phosphorylation pathway can be activated by cathepsin K inhibition (e.g., with odanacatib), leading to enhanced p53 stabilization and apoptosis sensitization. xref Conversely, phosphorylation at tyrosine 26 (Y26) by non-receptor tyrosine kinases (e.g., Src) induces distinct conformational changes that promote the specific stabilization of the mTORC1 component Raptor without affecting other substrates, demonstrating the high specificity of phosphorylation-mediated regulation. xref Additionally, the phosphorylated Y26 residue serves as a docking site for the cell cycle regulator p27, connecting OTUB1 to IFNγ-induced cell-cycle arrest. xref These mechanisms demonstrate how site-specific phosphorylation enables OTUB1 to process disparate upstream signals, such as DNA damage, and direct appropriate downstream responses."
41550777
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"One avenue might entail disruption of the inhibitory complex by phosphorylation of OTUB1 on one or more of the nine conserved phosphorylatable residues located at essential interaction surfaces."
22325355
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"Phosphorylated OTUB1 in the nucleus facilitates the repair of DNA damage induced by ionizing radiation (IR) by promoting 53BP1 DNA repair foci formation [ xref ]."
39062505
sparser
"Recently, we reported that phospho-OTUB1 and Raptor are overexpressed in RCC tumor tissues, and identified the positive correlation of between two proteins [ xref ]."
37330581
rlimsp
"Further, our study uncovers a signal-induced phosphorylation-dependent recruitment of OTUB1 to its target in the TGFβ pathway."
24071738
rlimsp
"We demonstrate that the Yersinia-encoded virulence factor YpkA (YopO) kinase interacts with a post-translationally modified form of OTUB1 that contains multiple phosphorylation sites."
20553488
sparser
"Src activates OTUB1 phosphorylation and p27 expression."
38664499
sparser
"As shown in Fig. xref , both Syk and ZAP70 knockdown decreased the phosphorylation of SHP2 (Y542) and increased the phosphorylation of OTUB1 (Y26) in cathepsin K siRNA-treated cells."
37330581
sparser
"Only CK2α, but not CaMK1α/β, NUAK1/2, GSK3β, Aurora A, B, and C and PLK1, phosphorylated OTUB1 robustly in vitro ( xref )."
25872870
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"Phosphorylation of OTUB1 at Ser 16 causes nuclear accumulation of OTUB1."
25872870
sparser
"In our study, a JAK2 inhibitor (AG490) attenuated the phosphorylation of OTUB1 (Tyr26) and p27 expression, with no impact from OTUB1, suggesting that JAK2 might serve as an upstream factor activating OTUB1-mediated p27 expression."
38664499
sparser
"In the first method, phosphorylated OTUB1 from the CK2α kinase assay using γ32 P-ATP in vitro was excised from the gel, trypsin-digested, and separated by chromatography on a C 18 column."
25872870
sparser
"In addition, the literature indicates that CK2 may regulate the stability of several proteins; for example, OTUB1 deubiquitinase phosphorylation promotes its nuclear activity, deubiquitinating and stabilizing chromatin binding proteins [ xref , xref , xref ]."
36766848
sparser
"Phosphorylation of OTUB1 at Ser 16 impacts IR-induced DNA-damage repair."
25872870
sparser
"Consistent with these findings, CK2α failed to phosphorylate OTUB1[S16A] and OTUB1[S16A/S18A] mutants but not OTUB1[S18A] in vitro ( xref )."
25872870
sparser
"Our current study reveals Src as a crucial component for the phosphorylation of OTUB1 (Tyr26) and p27, leading to cell cycle arrest in breast cancer cells."
38664499
sparser
"Furthermore, in our phosphoproteomic analysis of OTUB1 when overexpressed in HEK293 cells, we observed a two-fold increase in the phosphorylation of the identified OTUB1 phosphopeptide (QEPLG S D S EGVNCLAYDEAIMAQQDR) upon TGFβ stimulation ( xref )."
25872870
sparser
"Further, Seo et al. have recently shown that the knockdown of SRMS decreases the phosphorylation of OTU domain-containing ubiquitin aldehyde-binding proteins Otubain1 (OTUB1), resulting in the down-regulation of Regulatory-associated protein of TOR (Raptor) [ xref ]."
37251450
sparser
"YpkA is capable of phosphorylating otubain 1 in vitro only when actin is present to activate YpkA ( Fig. 4 A)."
16364312
sparser
"Several DUB enzymes are regulated by serine phosphorylation, including TNFAIP3 (A20) whose DUB activity is increased upon phosphorylation [57] , In contrast, serine phosphorylation of CYLD and OTUB1 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23333852
reach
"Our results demonstrate that cathepsin K inhibition enhances oxaliplatin-induced apoptosis by increasing OTUB1 phosphorylation via CK2 activation, thereby promoting p53 stabilization, and hence upregulating Bax."
34785775
sparser
"Figure xref E, xref demonstrated that both SHP2 inhibitor (SHP099) and SHP2 knockdown alone induced the increase of Raptor and phosphorylation of Src (Y416) and OTUB1 (Y26), whereas ODN treatment induced Raptor downregulation and dephosphorylation of Src (Y416) and OTUB1 (Y26)."
37330581
sparser
"OTUB1 is regulated by a more stringent posttranslational network due to its core deubiquitinase function, and the ubiquitination and phosphorylation of OTUB1 were studied in this paper."
34875341
sparser
"To investigate the molecular mechanisms underlying OTUB1 phosphorylation, we used the SHP2 catalytic-dead mutant (C459S) and Src catalytic-dead mutant (K295R and Y527F)."
37330581
sparser
"Next, we examined the role of tyrosine phosphatase in regulating OTUB1 phosphorylation and discovered that SHP2 knockdown prevented ODN-mediated Raptor downregulation (Fig. xref )."
37330581
sparser
"Interestingly, otubain 1 is phosphorylated in HeLa cells by an unknown serine/threonine kinase [11] , suggesting otubain 1 is regulated by phosphorylation in vivo."
16364312
sparser
"Our findings show that the phosphorylation of OTUB1 at Ser 16 did not impact its catalytic activity and its ability to interact with K63-linked ubiquitin chains as well as the E2 enzyme, UBE2N. Instead, we demonstrated that Ser 16 phosphorylation of OTUB1 was essential for its nuclear accumulation, suggesting that nuclear roles of OTUB1 are likely to be regulated through CK2-mediated phosphorylation of OTUB1."
25872870
sparser
"Until now, no reports showed whether and how these sites on OTUB1 were phosphorylated at the endogenous level."
25872870
sparser
"As otubain 1 has been shown to interact with the E3 ligase GRAIL, leading to GRAIL degradation and repression of T-cell anergy [12] , it is tempting to speculate that otubain 1 phosphorylation by YpkA[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16364312
sparser
"OTUB1 could also be phosphorylated by several serine/threonine kinases, previous studies have proved that OTUB1 includes multiple phosphorylation sites, such as Ser16, Ser 18 and Tyr26 [ 28–30 ]."
34875341
sparser
"Interestingly, depletion of SHP2 phosphorylated OTUB1 (Y26) and Src (Y416), but not OTUB1 (S16) (Fig. xref )."
37330581
sparser
"Current efforts directed towards examining all possible consequences of otubain 1 phosphorylation by YpkA should aid in understanding the molecular details of how this mechanism is advantageous for Ye[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16364312
sparser
"Furthermore, we detected high levels of phospho-OTUB1 and Raptor in samples of patients with renal clear carcinoma."
35927303
sparser
"This raises the possibility that CK2-mediated phosphorylation of OTUB1 is a regulated process."
25872870
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"Our results suggested that regulation of OTUB1 phosphorylation may be an effective and selective therapeutic target for treating cancers via down-regulation of Raptor."
35927303
sparser
"For example, phosphorylation of mammalian OTUB1, USP10, and ATXN3 results in their nuclear localization. xref – xref But how DUB activity is enhanced or inhibited by protein-protein interactions is poorly understood, especially in plants."
35700731
sparser
"Moreover, the Src mutant inhibited SHP2 mutant-induced OTUB1 phosphorylation and Raptor upregulation (Fig. xref ), indicating that Src activation is required for SHP2 mutant-mediated OTUB1 phosphorylation and Raptor upregulation."
37330581
sparser
"The phosphorylated OTU domain displays catalytic activity similar to the full length DUBA ( xref )."
30232733
sparser
"ALK2 through ALK6 all phosphorylated OTUB1 in vitro; however the TGFβ-activated ALK4 and ALK5 phosphorylated OTUB1 more robustly than the BMP-activated ALK2, ALK3 and ALK6 ( xref )."
25872870
sparser
"Additionally, we assessed the effects of other cytokines, such as TWEAK, LPS, TNF-α, and TGF-β, on OTUB1 phosphorylation (Tyr26) and p27 expression."
38664499
sparser
"In contrast, OTUB1[S18A] was poorly phosphorylated by ALK5, whereas OTUB1[S16A/S18A] was not phosphorylated ( xref ), suggesting that Ser 18 is the main ALK5 phosphorylation site on OTUB1."
25872870
sparser
"Literature shows that CK2 can regulate the stability of many other proteins, for example, OTUB1 phosphorylation promotes its nuclear deubiquitinase activity [ xref ]."
39443981
sparser
"Otubain 1 Phosphorylation Interferes with its Catalytic Activity and Function in Bacterial Infection."
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