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USP22 decreases the amount of ZEB1. 4 / 4
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"Our results showed that ectopic expression of USP22 increased ZEB1 protein expression and USP22 deletion reduced ZEB1 protein expression, while USP22 had no obvious effect on mRNA expression of ZEB1 in HCC-derived cell lines (Fig. 4A–C)."
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"USP22 depletion or USP22 deubiquitinase inactive mutant USP22 (m) enhanced the ubiquitination level of ZEB1 (Fig. 4H, I)."
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"The results showed that overexpression of USP22 also reduced the ubiquitination level of ZEB1 in HCCLM3 cells (Fig. 4J)."
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"The results demonstrated that the ubiquitination level of ZEB1 was significantly decreased by ectopic expression of USP22 in HCCLM3 and Huh7 cells (Fig. 4G and Supplementary Fig. S5B)."
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