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USP38 activates HIF1. 4 / 4
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"Ectopic expression of USP38 caused HIF1α stabilization in a dose-dependent manner (Fig. 3A)."
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"We further found that ectopic expression of USP38 also stabilized the HIF1α hydroxylation-deficient mutants, HIF1α (in which Pro 402 and 564, and Asp 803 were mutated to alanine) and HIF1α (in which Pro402 and 564 were mutated to alanine) (Fig. S5), indicating that USP38-mediated HIF1α stabilization is independent of PHD/VHL-mediated oxygen-dependent degradation of HIF1α."
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"Treatment with cycloheximide (CHX) indicated that USP38 significantly suppressed HIF1α degradation (Fig. 3D)."
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"To determine whether USP38-mediated HIF1α stabilization is dependent on the deubiquitinase activity of USP38, we used the enzymatically inactive mutants for subsequent assays (37, 38, 39)."
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