IndraLab
Statements
sparser
"Employing a stable cell line expressing FLAG-GFP-RETREG1 and utilizing FLAG for pulldown of the conjugated ubiquitin signal, we observed an increase in the conjugated ubiquitin signal on RETREG1 upon USP20 knockdown ( xref ), aligning with the data obtained from treating cells with the USP20 inhibitor GSK2643943A. Moreover, the immunoprecipitation assay demonstrated an interaction between MYC-USP20 and FLAG-RETREG1, indicating their potential binding capability ( xref )."